Detailed information of TA system TA203524

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3557050..3557670
• Replicon: chromosome
• Accession: NZ_CP075372
• Organism: Salmonella enterica strain no75

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: V1H8E6
• Locus tag: KH432_RS17575
• Protein ID: WP_001280991.1
• Coordinates: 3557452..3557670 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: V1H4V6
• Locus tag: KH432_RS17570
• Protein ID: WP_000344807.1
• Coordinates: 3557050..3557424 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KH432_RS17560 (3552189)	3552189..3553382	+	1194	WP_001039202.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
KH432_RS17565 (3553405)	3553405..3556554	+	3150	WP_001132508.1	efflux RND transporter permease AcrB	-
KH432_RS17570 (3557050)	3557050..3557424	+	375	WP_000344807.1	Hha toxicity modulator TomB	Antitoxin
KH432_RS17575 (3557452)	3557452..3557670	+	219	WP_001280991.1	HHA domain-containing protein	Toxin
KH432_RS17580 (3557849)	3557849..3558400	+	552	WP_001278792.1	maltose O-acetyltransferase	-
KH432_RS17585 (3558517)	3558517..3558987	+	471	WP_000136181.1	YlaC family protein	-
KH432_RS17590 (3559043)	3559043..3559183	-	141	WP_001197749.1	type B 50S ribosomal protein L36	-
KH432_RS17595 (3559189)	3559189..3559449	-	261	WP_000801415.1	type B 50S ribosomal protein L31	-
KH432_RS17600 (3559674)	3559674..3561224	+	1551	WP_000213139.1	EAL domain-containing protein	-
KH432_RS17610 (3561455)	3561455..3561844	+	390	WP_000961285.1	MGMT family protein	-
KH432_RS17615 (3561877)	3561877..3562446	-	570	WP_000779803.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	allD / allC / allB / allR / allA / allS / acrA / acrB	3486353..3734133	247780

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8613.97 Da
• Isoelectric Point: 8.9008

>T203524 WP_001280991.1 NZ_CP075372:3557452-3557670 [Salmonella enterica]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T203524 NZ_CP100110:c4857717-4857433 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14453.27 Da
• Isoelectric Point: 5.1444

>AT203524 WP_000344807.1 NZ_CP075372:3557050-3557424 [Salmonella enterica]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT203524 NZ_CP100110:c4857949-4857707 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• PDB: 4ICG

Antitoxin

• AlphaFold DB: A0A5I5FGI0