Detailed information of TA system TA203507

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 9032..9725
• Replicon: plasmid pPB07_4
• Accession: NZ_CP075358
• Organism: Pseudochrobactrum algeriensis isolate C130915_07

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: KHQ09_RS00045
• Protein ID: WP_213595987.1
• Coordinates: 9348..9725 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: KHQ09_RS00040
• Protein ID: WP_080921082.1
• Coordinates: 9032..9367 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHQ09_RS00020 (KHQ09_00020)	6311..6616	+	306	WP_213596001.1	mobilization protein	-
KHQ09_RS00025 (KHQ09_00025)	6613..8289	+	1677	WP_213595992.1	type IV secretory system conjugative DNA transfer family protein	-
KHQ09_RS00030 (KHQ09_00030)	8301..8690	+	390	WP_213595991.1	hypothetical protein	-
KHQ09_RS00035 (KHQ09_00035)	8814..9032	-	219	WP_213595989.1	hypothetical protein	-
KHQ09_RS00040 (KHQ09_00040)	9032..9367	-	336	WP_080921082.1	helix-turn-helix transcriptional regulator	Antitoxin
KHQ09_RS00045 (KHQ09_00045)	9348..9725	-	378	WP_213595987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KHQ09_RS00050 (KHQ09_00050)	9813..10133	-	321	WP_213596018.1	chromosome partitioning protein ParB	-
KHQ09_RS00055 (KHQ09_00055)	10130..10789	-	660	WP_080921084.1	ParA family protein	-
KHQ09_RS00060 (KHQ09_00060)	10868..11521	-	654	WP_213596016.1	SprT-like domain-containing protein	-
KHQ09_RS00065 (KHQ09_00065)	11764..12722	-	959	Protein_12	helix-turn-helix domain-containing protein	-
KHQ09_RS00070 (KHQ09_00070)	12916..13125	-	210	WP_025031070.1	ribbon-helix-helix protein, CopG family	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..21683	21683

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-97)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13956.02 Da
• Isoelectric Point: 8.0692

>T203507 WP_213595987.1 NZ_CP075358:c9725-9348 [Pseudochrobactrum algeriensis]
MNDDERPLEWIGSSYKDLMQLPADVRKFFGFALSFAQQGEQHAAAKVLKGFGGAGVLELVENDAGGTYRAVYTVKFEEAV
FVLHCFQKKSKSGIATPKADMDVIRARLKVAEIVAKEMRHENPHR

Nucleotide

• Length: 378 bp

>T203507 NZ_CP100106:c4745821-4745288 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12141.19 Da
• Isoelectric Point: 9.0961

>AT203507 WP_080921082.1 NZ_CP075358:c9367-9032 [Pseudochrobactrum algeriensis]
MRTRIVNGVEVTTSSGNVFADLGLPDAEKLKMKSGLMIEITKAVRRLDLTQEEAGRRMGLPQPKVSALLRGDFTNLSERK
LMECLNRLGYDIEITVKPAADAVGHMTLAVA

Nucleotide

• Length: 336 bp

>AT203507 NZ_CP100106:c4746098-4745832 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA