Detailed information of TA system TA203506

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 1065..1758
• Replicon: plasmid pPB16_4
• Accession: NZ_CP075354
• Organism: Pseudochrobactrum algeriensis strain C150915_16

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: KHQ07_RS00180
• Protein ID: WP_213595987.1
• Coordinates: 1065..1442 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: KHQ07_RS00185
• Protein ID: WP_080921082.1
• Coordinates: 1423..1758 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHQ07_RS00170 (KHQ07_00170)	1..660	+	660	WP_080921084.1	ParA family protein	-
KHQ07_RS00175 (KHQ07_00175)	657..977	+	321	WP_213596018.1	chromosome partitioning protein ParB	-
KHQ07_RS00180 (KHQ07_00180)	1065..1442	+	378	WP_213595987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KHQ07_RS00185 (KHQ07_00185)	1423..1758	+	336	WP_080921082.1	helix-turn-helix transcriptional regulator	Antitoxin
KHQ07_RS00190 (KHQ07_00190)	1758..1976	+	219	WP_213595989.1	hypothetical protein	-
KHQ07_RS00195 (KHQ07_00195)	2100..2489	-	390	WP_213595991.1	hypothetical protein	-
KHQ07_RS00200 (KHQ07_00200)	2501..4177	-	1677	WP_213595992.1	type IV secretory system conjugative DNA transfer family protein	-
KHQ07_RS00205 (KHQ07_00205)	4174..4479	-	306	WP_213596001.1	mobilization protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..21732	21732

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-97)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13956.02 Da
• Isoelectric Point: 8.0692

>T203506 WP_213595987.1 NZ_CP075354:1065-1442 [Pseudochrobactrum algeriensis]
MNDDERPLEWIGSSYKDLMQLPADVRKFFGFALSFAQQGEQHAAAKVLKGFGGAGVLELVENDAGGTYRAVYTVKFEEAV
FVLHCFQKKSKSGIATPKADMDVIRARLKVAEIVAKEMRHENPHR

Nucleotide

• Length: 378 bp

>T203506 NZ_CP100106:4109717-4109935 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12141.19 Da
• Isoelectric Point: 9.0961

>AT203506 WP_080921082.1 NZ_CP075354:1423-1758 [Pseudochrobactrum algeriensis]
MRTRIVNGVEVTTSSGNVFADLGLPDAEKLKMKSGLMIEITKAVRRLDLTQEEAGRRMGLPQPKVSALLRGDFTNLSERK
LMECLNRLGYDIEITVKPAADAVGHMTLAVA

Nucleotide

• Length: 336 bp

>AT203506 NZ_CP100106:4109316-4109690 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG