Detailed information of TA system TA203503

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3797860..3798575
• Replicon: chromosome
• Accession: NZ_CP075345
• Organism: Enterobacter kobei strain S18_CRE18

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A8I0UM05
• Locus tag: KJE18_RS18195
• Protein ID: WP_023303149.1
• Coordinates: 3797860..3798228 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A8I0UJV2
• Locus tag: KJE18_RS18200
• Protein ID: WP_023303148.1
• Coordinates: 3798249..3798575 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KJE18_RS18155	3792883..3793311	+	429	WP_032638336.1	hypothetical protein	-
KJE18_RS18160 (KJE18_18120)	3793287..3793850	+	564	Protein_3556	IS3 family transposase	-
KJE18_RS18165 (KJE18_18125)	3794005..3794868	+	864	WP_001065553.1	GTPase family protein	-
KJE18_RS18170 (KJE18_18130)	3794960..3795781	+	822	WP_000197389.1	DUF932 domain-containing protein	-
KJE18_RS18175 (KJE18_18135)	3795998..3796699	+	702	WP_006812588.1	WYL domain-containing protein	-
KJE18_RS18180 (KJE18_18140)	3796740..3796976	+	237	WP_001144031.1	protein YpjK	-
KJE18_RS18185 (KJE18_18145)	3796976..3797419	+	444	WP_000824223.1	lipoprotein YafY	-
KJE18_RS18190	3797443..3797538	+	96	Protein_3562	hypothetical protein	-
KJE18_RS18195 (KJE18_18150)	3797860..3798228	-	369	WP_023303149.1	TA system toxin CbtA family protein	Toxin
KJE18_RS18200 (KJE18_18155)	3798249..3798575	-	327	WP_023303148.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KJE18_RS18205 (KJE18_18160)	3798588..3799058	-	471	WP_023303147.1	DNA repair protein RadC	-
KJE18_RS18210 (KJE18_18165)	3799124..3799945	-	822	WP_023303146.1	DUF932 domain-containing protein	-
KJE18_RS18215 (KJE18_18170)	3800635..3801474	-	840	WP_023303145.1	hypothetical protein	-
KJE18_RS18220 (KJE18_18175)	3801533..3801967	-	435	WP_023303144.1	hypothetical protein	-
KJE18_RS18225 (KJE18_18180)	3802276..3803541	-	1266	WP_023303143.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3793308..3819494	26186
	-	flank	IS/Tn	-	-	3793308..3793850	542

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (7-107)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13727.91 Da
• Isoelectric Point: 8.2822

>T203503 WP_023303149.1 NZ_CP075345:c3798228-3797860 [Enterobacter kobei]
MKNLPATISRAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGIILADAVNFLVDKYELVRIDRRGF
SSQGQVPYLTVTDILHARRACGLMKSCSYREVSNIVLSHSRQ

Nucleotide

• Length: 369 bp

>T203503 NZ_CP100106:2024675-2024777 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12215.04 Da
• Isoelectric Point: 7.0266

>AT203503 WP_023303148.1 NZ_CP075345:c3798575-3798249 [Enterobacter kobei]
MSLIPAHEWGIKSDIVPRLGVRLVQEGNRLHYLADRASITGKFSDAECLKLDIVFPHFIRQMESMLTTGEMNSRHAHCVT
LYHNGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 327 bp

>AT203503 NZ_CP100106:c2024784-2024639 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT