Detailed information of TA system TA020350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 546156..546774
• Replicon: chromosome
• Accession: NC_002695
• Organism: Escherichia coli O157:H7 str. Sakai

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: ECs_0513
• Protein ID: NP_308540.1
• Coordinates: 546156..546374 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: ECs_0514
• Protein ID: NP_308541.1
• Coordinates: 546400..546774 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECs_0507	541443..542015	+	573	NP_308534.3	outer membrane lipoprotein	-
ECs_0508	542048..542359	-	312	NP_308535.2	6-O-methylguanine DNA methyltransferase	-
ECs_0509	542738..543091	+	354	NP_308536.1	hypothetical protein	-
ECs_0510	543133..544683	-	1551	NP_308537.2	membrane-anchored cyclic-di-GMP phosphodiesterase	-
ECs_0511	544847..545317	-	471	NP_308538.2	hypothetical protein	-
ECs_0512	545433..545984	-	552	NP_308539.1	maltose O-acetyltransferase	-
ECs_0513	546156..546374	-	219	NP_308540.1	modulator of gene expression with H-NS	Toxin
ECs_0514	546400..546774	-	375	NP_308541.1	Hha toxicity attenuator TomB	Antitoxin
ECs_0515	547320..550469	-	3150	NP_308542.1	multidrug efflux system protein	-
ECs_0516	550492..551685	-	1194	NP_308543.1	multidrug efflux system	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T20350 NP_308540.1 NC_002695:c546374-546156 [Escherichia coli O157:H7 str. Sakai]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T20350 NC_002695:c546374-546156 [Escherichia coli O157:H7 str. Sakai]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT20350 NP_308541.1 NC_002695:c546774-546400 [Escherichia coli O157:H7 str. Sakai]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT20350 NC_002695:c546774-546400 [Escherichia coli O157:H7 str. Sakai]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG