Detailed information of TA system TA203466

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 518581..519217
• Replicon: chromosome
• Accession: NZ_CP075344
• Organism: Bacillus subtilis subsp. subtilis strain A1 - Midalam

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: KIL00_RS02630
• Protein ID: WP_003156187.1
• Coordinates: 518867..519217 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: KIL00_RS02625
• Protein ID: WP_003225183.1
• Coordinates: 518581..518862 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIL00_RS02605 (514940)	514940..515539	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
KIL00_RS02610 (515634)	515634..515999	+	366	WP_014478897.1	holo-ACP synthase	-
KIL00_RS02615 (516165)	516165..517181	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
KIL00_RS02620 (517296)	517296..518465	+	1170	WP_014478898.1	alanine racemase	-
KIL00_RS02625 (518581)	518581..518862	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
KIL00_RS02630 (518867)	518867..519217	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
KIL00_RS02635 (519333)	519333..520157	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
KIL00_RS02640 (520162)	520162..520527	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
KIL00_RS02645 (520531)	520531..520932	+	402	WP_014478899.1	serine/threonine-protein kinase RsbT	-
KIL00_RS02650 (520944)	520944..521951	+	1008	WP_014478900.1	phosphoserine phosphatase RsbU	-
KIL00_RS02655 (522020)	522020..522349	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
KIL00_RS02660 (522346)	522346..522828	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
KIL00_RS02665 (522794)	522794..523582	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
KIL00_RS02670 (523582)	523582..524181	+	600	WP_003246608.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T203466 WP_003156187.1 NZ_CP075344:518867-519217 [Bacillus subtilis subsp. subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T203466 NZ_CP100096:c6145-5840 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT203466 WP_003225183.1 NZ_CP075344:518581-518862 [Bacillus subtilis subsp. subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT203466 NZ_CP100096:c6365-6147 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: G4NU32