Detailed information of TA system TA203465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2528257..2528821
• Replicon: chromosome
• Accession: NZ_CP075343
• Organism: Streptomyces sp. EMB24

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KIH30_RS11320
• Protein ID: WP_102640794.1
• Coordinates: 2528257..2528604 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: KIH30_RS11325
• Protein ID: WP_102640795.1
• Coordinates: 2528591..2528821 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIH30_RS11305	2525472..2526314	-	843	WP_199208621.1	hypothetical protein	-
KIH30_RS11310	2526316..2526849	-	534	WP_102640792.1	sigma-70 family RNA polymerase sigma factor	-
KIH30_RS11315	2527183..2528199	+	1017	WP_199208620.1	aspartate-semialdehyde dehydrogenase	-
KIH30_RS11320	2528257..2528604	-	348	WP_102640794.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KIH30_RS11325	2528591..2528821	-	231	WP_102640795.1	ribbon-helix-helix domain-containing protein	Antitoxin
KIH30_RS11330	2529015..2531621	+	2607	WP_268219859.1	aminopeptidase N	-
KIH30_RS11335	2531677..2532027	-	351	Protein_2241	TetR/AcrR family transcriptional regulator	-
KIH30_RS11340	2532078..2532275	-	198	WP_268219860.1	DUF397 domain-containing protein	-
KIH30_RS11345	2532284..2533135	-	852	WP_268219861.1	helix-turn-helix transcriptional regulator	-
KIH30_RS11350	2533338..2533742	+	405	Protein_2244	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-38)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12667.55 Da
• Isoelectric Point: 10.9360

>T203465 WP_102640794.1 NZ_CP075343:c2528604-2528257 [Streptomyces sp. EMB24]
MRRGDIYMVDLEPVRGSEANKVRPAVIVSNDGANASVERHGRGVVSVVPLTSNTTRVLSFQVFLSADESRLPKDSKAQCE
QVRAVAPDRLLHRVGAVPRQRMADIDVALRRHLAL

Nucleotide

• Length: 348 bp

>T203465 NZ_CP100095:181296-181712 [Klebsiella pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8345.04 Da
• Isoelectric Point: 3.7832

>AT203465 WP_102640795.1 NZ_CP075343:c2528821-2528591 [Streptomyces sp. EMB24]
MKISVSLPQEDVAFVDEYAARTDAESRSAVIHAAIELLRQAQLVQEYTEAFAEWDAGEDAAAWDRTSGDGVADEAW

Nucleotide

• Length: 231 bp

>AT203465 NZ_CP100095:181069-181299 [Klebsiella pneumoniae]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGACGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA