Detailed information of TA system TA203463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 4608293..4608858
• Replicon: chromosome
• Accession: NZ_CP075340
• Organism: Rhodococcus ruber strain ZM15

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A385HTA4
• Locus tag: KI427_RS21090
• Protein ID: WP_010593326.1
• Coordinates: 4608293..4608649 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A385HSN3
• Locus tag: KI427_RS21095
• Protein ID: WP_010593327.1
• Coordinates: 4608646..4608858 (-)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI427_RS21065 (KI427_21045)	4604507..4605004	-	498	WP_119700016.1	hypothetical protein	-
KI427_RS21070 (KI427_21050)	4605368..4606426	-	1059	WP_010593322.1	mannan endo-1,4-beta-mannosidase	-
KI427_RS21075 (KI427_21055)	4606613..4606861	+	249	WP_029539937.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
KI427_RS21080 (KI427_21060)	4606846..4607238	+	393	WP_010593324.1	type II toxin-antitoxin system VapC family toxin	-
KI427_RS21085 (KI427_21065)	4607400..4608230	+	831	WP_244213176.1	hypothetical protein	-
KI427_RS21090 (KI427_21070)	4608293..4608649	-	357	WP_010593326.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KI427_RS21095 (KI427_21075)	4608646..4608858	-	213	WP_010593327.1	ribbon-helix-helix protein, CopG family	Antitoxin
KI427_RS21100 (KI427_21080)	4609069..4609650	+	582	WP_010593328.1	GNAT family N-acetyltransferase	-
KI427_RS21105 (KI427_21085)	4609658..4610104	-	447	WP_237658121.1	hypothetical protein	-
KI427_RS21110 (KI427_21090)	4610177..4611460	+	1284	WP_029539938.1	competence/damage-inducible protein A	-
KI427_RS21115 (KI427_21095)	4611905..4612816	+	912	WP_029539939.1	formylglycine-generating enzyme family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-114)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-40)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12867.56 Da
• Isoelectric Point: 7.3229

>T203463 WP_010593326.1 NZ_CP075340:c4608649-4608293 [Rhodococcus ruber]
MIRRAQIHWVDPDPAQGSEAAERRPAVVVSNDRANATAENLGRGVVTVVPLTTNTSHVFPFQVLVEPDESGLTRAAKAQA
EQVRSVSVRRLDTEPVGRLGARTLAALEEALRLHLDLR

Nucleotide

• Length: 357 bp

>T203463 NZ_CP100095:53922-54404 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7652.40 Da
• Isoelectric Point: 4.1694

>AT203463 WP_010593327.1 NZ_CP075340:c4608858-4608646 [Rhodococcus ruber]
MTVKFTISLPDEDAHVLDEYIRDHGLRGRSAGVRAAVRLLAAAGLERDYATAFAEVDTDDAWDVTTADTE

Nucleotide

• Length: 213 bp

>AT203463 NZ_CP100095:53668-53934 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A385HTA4

Antitoxin

• AlphaFold DB: A0A385HSN3