Detailed information of TA system TA020346

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 1748433..1749028
• Replicon: chromosome
• Accession: NC_002678
• Organism: Mesorhizobium loti MAFF303099

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: MAFF_RS37580
• Protein ID: WP_010910700.1
• Coordinates: 1748433..1748621 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q98J17
• Locus tag: MAFF_RS09595
• Protein ID: WP_010910701.1
• Coordinates: 1748627..1749028 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAFF_RS09575	1743740..1744222	+	483	WP_157866158.1	flagellar basal body-associated protein FliL	-
MAFF_RS09580	1744257..1745273	-	1017	WP_010910697.1	ABC transporter permease	-
MAFF_RS09585	1745270..1746883	-	1614	WP_010910698.1	sugar ABC transporter ATP-binding protein	-
MAFF_RS09590	1747126..1748073	-	948	WP_044548186.1	substrate-binding domain-containing protein	-
MAFF_RS37580	1748433..1748621	+	189	WP_010910700.1	type II toxin-antitoxin system HicA family toxin	Toxin
MAFF_RS09595	1748627..1749028	+	402	WP_010910701.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
MAFF_RS09600	1749065..1749763	+	699	WP_010910702.1	RibD family protein	-
MAFF_RS09605	1749776..1750660	-	885	WP_010910703.1	SDR family oxidoreductase	-
MAFF_RS09610	1750751..1751581	+	831	WP_010910704.1	helix-turn-helix transcriptional regulator	-
MAFF_RS09615	1751610..1751987	-	378	WP_157866159.1	SRPBCC domain-containing protein	-
MAFF_RS09620	1752438..1753733	+	1296	WP_010910706.1	TIGR01244 family phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7118.20 Da
• Isoelectric Point: 11.6118

>T20346 WP_010910700.1 NC_002678:1748433-1748621 [Mesorhizobium japonicum MAFF 303099]
MNSAEIIRQLQADGWFEVARKGSHAQFKHRDKQGRVTVPHPKRDIPIGTLRSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T20346 NC_002678:1748433-1748621 [Mesorhizobium japonicum MAFF 303099]
ATGAATAGCGCCGAAATTATCCGCCAGTTGCAAGCTGATGGTTGGTTCGAGGTTGCCCGAAAGGGCAGCCATGCGCAATT
CAAACACCGTGACAAGCAAGGGCGGGTCACCGTCCCTCATCCCAAGCGCGATATCCCAATAGGCACTCTACGCAGCATCG
AAAAGCAATCTGGACTGAAACTGAGGTGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14185.00 Da
• Isoelectric Point: 4.3919

>AT20346 WP_010910701.1 NC_002678:1748627-1749028 [Mesorhizobium japonicum MAFF 303099]
MKQYIGLIHKDAGSDFGVSFPDFPGVITAGKDLDDARSMAEEALTLHIEGLVEDGEAIPEPSSLQAVMADADNRDGVAIL
VAAKTEARKAVRINVTLPDDVLRRIDAFAEAHGYTRSGFLAKAAEKAMELESA

Nucleotide

• Length: 402 bp

>AT20346 NC_002678:1748627-1749028 [Mesorhizobium japonicum MAFF 303099]
ATGAAGCAGTATATCGGACTTATCCATAAGGATGCCGGCAGCGACTTCGGTGTGTCTTTCCCTGATTTCCCAGGTGTGAT
TACCGCCGGAAAGGATCTGGATGACGCACGGTCCATGGCTGAAGAGGCGCTCACCCTCCACATCGAAGGGCTGGTCGAGG
ATGGCGAAGCCATTCCGGAGCCTTCCAGTCTGCAGGCCGTGATGGCGGACGCCGATAATCGTGACGGTGTCGCAATCCTG
GTCGCGGCCAAGACCGAGGCCAGGAAGGCGGTGCGTATCAACGTGACGTTGCCAGACGACGTTCTGCGGCGAATCGATGC
CTTTGCCGAAGCCCACGGCTATACGCGATCCGGGTTTCTCGCAAAGGCGGCTGAGAAAGCGATGGAGCTTGAATCGGCCT
GA

Structures

Antitoxin

• AlphaFold DB: A0A4Q1UK54