Detailed information of TA system TA203459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 21157..21746
• Replicon: plasmid pXAC33
• Accession: NZ_CP075327
• Organism: Xanthomonas citri pv. citri strain GD82

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: M4U123
• Locus tag: KJA71_RS23620
• Protein ID: WP_015463824.1
• Coordinates: 21157..21489 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: M4UHU6
• Locus tag: KJA71_RS23625
• Protein ID: WP_011052949.1
• Coordinates: 21489..21746 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KJA71_RS23600 (KJA71_23600)	19637..19858	-	222	WP_011052944.1	hypothetical protein	-
KJA71_RS23605 (KJA71_23605)	20148..20684	-	537	WP_015463823.1	plasmid pRiA4b ORF-3 family protein	-
KJA71_RS23610 (KJA71_23610)	20674..20886	-	213	WP_011052947.1	hypothetical protein	-
KJA71_RS23615 (KJA71_23615)	20883..21134	-	252	WP_125459571.1	hypothetical protein	-
KJA71_RS23620 (KJA71_23620)	21157..21489	-	333	WP_015463824.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KJA71_RS23625 (KJA71_23625)	21489..21746	-	258	WP_011052949.1	antitoxin	Antitoxin
KJA71_RS23630 (KJA71_23630)	21952..22554	+	603	WP_011052950.1	recombinase family protein	-
KJA71_RS23635 (KJA71_23635)	22538..22842	+	305	Protein_33	Tn3 family transposase	-
KJA71_RS23640 (KJA71_23640)	23009..23215	-	207	Protein_34	Tn3 family transposase	-
KJA71_RS23645 (KJA71_23645)	23260..23829	-	570	Protein_35	UPF0149 family protein	-
KJA71_RS23650 (KJA71_23650)	23848..25062	-	1215	WP_011050083.1	IS4-like element ISXac1 family transposase	-
KJA71_RS23655 (KJA71_23655)	25178..25318	-	141	Protein_37	UPF0149 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..34427	34427

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11707.46 Da
• Isoelectric Point: 9.1975

>T203459 WP_015463824.1 NZ_CP075327:c21489-21157 [Xanthomonas citri pv. citri]
MDRGDVYLVSLDPTSGHEQQGTRPVLIVSPSAFNRLTKTPVVLPITSGGNFARTAGFTVSLMGAGTNTTGVVRCDQPRAL
DLASRRARKLETVPSPIMDEVLAKLSAILE

Nucleotide

• Length: 333 bp

>T203459 NZ_CP100094:c4745491-4744958 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9242.70 Da
• Isoelectric Point: 4.7164

>AT203459 WP_011052949.1 NZ_CP075327:c21746-21489 [Xanthomonas citri pv. citri]
MHTTNLRKVGGSIMLAVPPAILDMLRLRAGATVGLAVDHGRLVIEPTLRPYYSLDELLAQCDASAELSTEDREWLDAKPV
GSELL

Nucleotide

• Length: 258 bp

>AT203459 NZ_CP100094:c4745768-4745502 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: M4U123

Antitoxin

• AlphaFold DB: M4UHU6