Detailed information of TA system TA020345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 1401426..1402099
• Replicon: chromosome
• Accession: NC_002678
• Organism: Mesorhizobium loti MAFF303099

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MAFF_RS07795
• Protein ID: WP_010910345.1
• Coordinates: 1401426..1401824 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q98K21
• Locus tag: MAFF_RS07800
• Protein ID: WP_010910346.1
• Coordinates: 1401824..1402099 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAFF_RS07770	1396583..1397314	+	732	WP_010910340.1	M15 family metallopeptidase	-
MAFF_RS07775	1397519..1398547	+	1029	WP_010910341.1	sulfate ABC transporter substrate-binding protein	-
MAFF_RS07780	1398629..1399480	+	852	WP_010910342.1	sulfate ABC transporter permease subunit CysT	-
MAFF_RS07785	1399473..1400357	+	885	WP_010910343.1	sulfate ABC transporter permease subunit CysW	-
MAFF_RS07790	1400370..1401410	+	1041	WP_010910344.1	sulfate/molybdate ABC transporter ATP-binding protein	-
MAFF_RS07795	1401426..1401824	-	399	WP_010910345.1	type II toxin-antitoxin system VapC family toxin	Toxin
MAFF_RS07800	1401824..1402099	-	276	WP_010910346.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MAFF_RS07805	1402324..1403310	+	987	WP_010910347.1	sulfate ABC transporter substrate-binding protein	-
MAFF_RS07810	1403559..1404008	+	450	WP_010910348.1	Rrf2 family transcriptional regulator	-
MAFF_RS07815	1404092..1404640	-	549	WP_044548091.1	gluconokinase	-
MAFF_RS07820	1404757..1405743	-	987	WP_032930880.1	ABC transporter permease	-
MAFF_RS07825	1405740..1406720	-	981	WP_010910351.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

• SpoVT-AbrB domain (18-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14274.67 Da
• Isoelectric Point: 5.2164

>T20345 WP_010910345.1 NC_002678:c1401824-1401426 [Mesorhizobium japonicum MAFF 303099]
MRFMLDTNIISDMIRNPAGKAAGAMVREGDAAVCTSIVVASELRYGCARKGSTKLLKKVEDLLAEIPVLPLDVPVDAEYG
ALRAELEAVGQPIGYNDLFIAAHACVLGTTLVTANIGEFTRIRKLKVENWLE

Nucleotide

• Length: 399 bp

>T20345 NC_002678:c1401824-1401426 [Mesorhizobium japonicum MAFF 303099]
ATGCGGTTCATGCTGGATACCAACATCATCAGCGACATGATCCGGAACCCGGCCGGCAAGGCAGCCGGTGCAATGGTGCG
CGAGGGAGACGCCGCTGTATGCACCAGCATCGTCGTTGCCTCTGAATTGCGATATGGCTGCGCCAGGAAGGGATCGACCA
AGCTGCTCAAGAAGGTCGAGGACCTGCTGGCCGAGATCCCCGTCCTGCCGCTCGACGTGCCGGTGGATGCCGAGTACGGC
GCACTTCGCGCCGAGCTGGAGGCTGTTGGCCAACCCATTGGCTACAATGACCTTTTTATTGCTGCTCACGCCTGCGTATT
GGGCACCACGCTGGTGACGGCCAACATCGGGGAGTTCACCCGGATACGGAAGCTGAAGGTCGAGAACTGGCTGGAATAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10230.68 Da
• Isoelectric Point: 5.1585

>AT20345 WP_010910346.1 NC_002678:c1402099-1401824 [Mesorhizobium japonicum MAFF 303099]
MPQHRHTTTPPPKEAKLFRNNRSQAVRIPVEFELPGDKVLISREGDRLVIEPLRKPGLAALLAQWAKEAPLGPEDNFPEI
NDAPVEAEDIF

Nucleotide

• Length: 276 bp

>AT20345 NC_002678:c1402099-1401824 [Mesorhizobium japonicum MAFF 303099]
ATGCCCCAGCATCGCCACACGACAACGCCACCACCGAAGGAGGCGAAACTGTTCCGAAACAACCGCAGCCAGGCGGTGCG
CATTCCTGTCGAGTTCGAACTCCCCGGCGACAAGGTTCTGATCAGCCGCGAAGGCGACCGCCTTGTCATTGAGCCGCTCC
GCAAACCTGGGCTTGCCGCGCTGCTCGCCCAATGGGCGAAAGAGGCGCCGCTTGGTCCTGAGGACAACTTTCCAGAGATC
AATGATGCGCCTGTCGAAGCCGAGGACATTTTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A4Q1UU16