Detailed information of TA system TA203448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 2308..2888
• Replicon: plasmid pDD521.4
• Accession: NZ_CP075320
• Organism: Klebsiella pneumoniae strain DD521

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: KJA60_RS27855
• Protein ID: WP_071177730.1
• Coordinates: 2574..2888 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: KJA60_RS27850
• Protein ID: WP_000093040.1
• Coordinates: 2308..2586 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KJA60_RS27835 (KJA60_27840)	881..1126	+	246	WP_032440458.1	hypothetical protein	-
KJA60_RS27840 (KJA60_27845)	1400..1771	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
KJA60_RS28070	1768..2133	+	366	WP_072354022.1	TonB family protein	-
KJA60_RS27850 (KJA60_27855)	2308..2586	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
KJA60_RS27855 (KJA60_27860)	2574..2888	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KJA60_RS27860 (KJA60_27865)	3052..3480	+	429	WP_001140599.1	hypothetical protein	-
KJA60_RS27865 (KJA60_27870)	3506..3685	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
KJA60_RS27870 (KJA60_27875)	3712..4242	-	531	WP_071177729.1	hypothetical protein	-
KJA60_RS27875 (KJA60_27880)	4249..4980	-	732	WP_071177728.1	MobC family replication-relaxation protein	-
KJA60_RS27880 (KJA60_27885)	4980..6944	-	1965	WP_071177727.1	TraM recognition domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11969	11969

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T203448 WP_071177730.1 NZ_CP075320:2574-2888 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T203448 NZ_CP100089:53873-54289 [Klebsiella pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT203448 WP_000093040.1 NZ_CP075320:2308-2586 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT203448 NZ_CP100089:53646-53876 [Klebsiella pneumoniae]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGACGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1