Detailed information of TA system TA203446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 5888..6413
• Replicon: plasmid pDD521.2
• Accession: NZ_CP075318
• Organism: Klebsiella pneumoniae strain DD521

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D4HQE7
• Locus tag: KJA60_RS26990
• Protein ID: WP_013023785.1
• Coordinates: 5888..6193 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W8V2V6
• Locus tag: KJA60_RS26995
• Protein ID: WP_001568025.1
• Coordinates: 6195..6413 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KJA60_RS26960 (1551)	1551..2177	+	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-
KJA60_RS28050 (2174)	2174..2476	+	303	WP_004197636.1	hypothetical protein	-
KJA60_RS26970 (2964)	2964..3758	-	795	WP_004197635.1	site-specific integrase	-
KJA60_RS26975 (3956)	3956..4972	-	1017	WP_017899884.1	hypothetical protein	-
KJA60_RS26980 (4983)	4983..5297	-	315	WP_053389906.1	hypothetical protein	-
KJA60_RS26985 (5324)	5324..5719	-	396	WP_017899885.1	hypothetical protein	-
KJA60_RS26990 (5888)	5888..6193	-	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	Toxin
KJA60_RS26995 (6195)	6195..6413	-	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
KJA60_RS27000 (6583)	6583..7044	-	462	WP_160880186.1	hypothetical protein	-
KJA60_RS27005 (7001)	7001..7231	+	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	-
KJA60_RS27010 (7228)	7228..7644	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	-
KJA60_RS27015 (7718)	7718..9280	+	1563	WP_004206609.1	AAA family ATPase	-
KJA60_RS27020 (9265)	9265..10287	+	1023	WP_000361404.1	helicase UvrD	-
KJA60_RS27025 (10543)	10543..11240	+	698	WP_223174630.1	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2	-	1..105939	105939

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11615.30 Da
• Isoelectric Point: 6.4672

>T203446 WP_013023785.1 NZ_CP075318:c6193-5888 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T203446 NZ_CP100088:82681-83097 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8392.34 Da
• Isoelectric Point: 4.7405

>AT203446 WP_001568025.1 NZ_CP075318:c6413-6195 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT203446 NZ_CP100088:82454-82684 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A2J4ZZW6

Antitoxin

• AlphaFold DB: A0A2J4ZZP8