Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5888..6413 Replicon plasmid pDD521.2
Accession NZ_CP075318
Organism Klebsiella pneumoniae strain DD521

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag KJA60_RS26990 Protein ID WP_013023785.1
Coordinates 5888..6193 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag KJA60_RS26995 Protein ID WP_001568025.1
Coordinates 6195..6413 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJA60_RS26960 (1551) 1551..2177 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
KJA60_RS28050 (2174) 2174..2476 + 303 WP_004197636.1 hypothetical protein -
KJA60_RS26970 (2964) 2964..3758 - 795 WP_004197635.1 site-specific integrase -
KJA60_RS26975 (3956) 3956..4972 - 1017 WP_017899884.1 hypothetical protein -
KJA60_RS26980 (4983) 4983..5297 - 315 WP_053389906.1 hypothetical protein -
KJA60_RS26985 (5324) 5324..5719 - 396 WP_017899885.1 hypothetical protein -
KJA60_RS26990 (5888) 5888..6193 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
KJA60_RS26995 (6195) 6195..6413 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KJA60_RS27000 (6583) 6583..7044 - 462 WP_160880186.1 hypothetical protein -
KJA60_RS27005 (7001) 7001..7231 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin -
KJA60_RS27010 (7228) 7228..7644 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -
KJA60_RS27015 (7718) 7718..9280 + 1563 WP_004206609.1 AAA family ATPase -
KJA60_RS27020 (9265) 9265..10287 + 1023 WP_000361404.1 helicase UvrD -
KJA60_RS27025 (10543) 10543..11240 + 698 WP_223174630.1 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 - 1..105939 105939


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T203446 WP_013023785.1 NZ_CP075318:c6193-5888 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T203446 NZ_CP100088:82681-83097 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT203446 WP_001568025.1 NZ_CP075318:c6413-6195 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT203446 NZ_CP100088:82454-82684 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References