Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5405522..5406147 Replicon chromosome
Accession NZ_CP075316
Organism Klebsiella pneumoniae strain DD521

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KJA60_RS25970 Protein ID WP_002882817.1
Coordinates 5405522..5405905 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KJA60_RS25975 Protein ID WP_004150355.1
Coordinates 5405905..5406147 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJA60_RS25955 (5402888) 5402888..5403790 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KJA60_RS25960 (5403787) 5403787..5404422 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KJA60_RS25965 (5404419) 5404419..5405348 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KJA60_RS25970 (5405522) 5405522..5405905 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KJA60_RS25975 (5405905) 5405905..5406147 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KJA60_RS25980 (5406352) 5406352..5407269 + 918 WP_004181612.1 alpha/beta hydrolase -
KJA60_RS25985 (5407283) 5407283..5408224 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KJA60_RS25990 (5408269) 5408269..5408706 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KJA60_RS25995 (5408703) 5408703..5409563 - 861 WP_004146232.1 virulence factor BrkB family protein -
KJA60_RS26000 (5409557) 5409557..5410156 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T203445 WP_002882817.1 NZ_CP075316:c5405905-5405522 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T203445 NZ_CP100088:c81646-81341 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT203445 WP_004150355.1 NZ_CP075316:c5406147-5405905 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT203445 NZ_CP100088:c81866-81648 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References