Detailed information of TA system TA203444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 5092414..5094095
• Replicon: chromosome
• Accession: NZ_CP075316
• Organism: Klebsiella pneumoniae strain DD521

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: KJA60_RS24525
• Protein ID: WP_004178299.1
• Coordinates: 5092414..5093331 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: KJA60_RS24530
• Protein ID: WP_004177809.1
• Coordinates: 5093337..5094095 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KJA60_RS24505 (5087720)	5087720..5089240	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
KJA60_RS24510 (5089237)	5089237..5090235	+	999	WP_002885172.1	ribose ABC transporter permease	-
KJA60_RS24515 (5090266)	5090266..5091207	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
KJA60_RS24520 (5091282)	5091282..5092289	+	1008	WP_004178300.1	carbohydrate kinase family protein	-
KJA60_RS24525 (5092414)	5092414..5093331	-	918	WP_004178299.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
KJA60_RS24530 (5093337)	5093337..5094095	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
KJA60_RS24535 (5094181)	5094181..5094909	-	729	WP_004178298.1	response regulator transcription factor	-
KJA60_RS24540 (5095182)	5095182..5097329	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
KJA60_RS24545 (5097326)	5097326..5097469	-	144	WP_022631365.1	hypothetical protein	-
KJA60_RS24550 (5097907)	5097907..5098593	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34649.06 Da
• Isoelectric Point: 5.4707

>T203444 WP_004178299.1 NZ_CP075316:c5093331-5092414 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPSQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T203444 NZ_CP100087:c5328429-5327896 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT203444 WP_004177809.1 NZ_CP075316:c5094095-5093337 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT203444 NZ_CP100087:c5328706-5328440 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A6TH05