Detailed information of TA system TA203439

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2909814..2911402
• Replicon: chromosome
• Accession: NZ_CP075316
• Organism: Klebsiella pneumoniae strain DD521

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: KJA60_RS14175
• Protein ID: WP_004179815.1
• Coordinates: 2909814..2911136 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: KJA60_RS14180
• Protein ID: WP_004176198.1
• Coordinates: 2911136..2911402 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KJA60_RS14160 (2904870)	2904870..2907371	+	2502	WP_020323802.1	fimbria/pilus outer membrane usher protein	-
KJA60_RS14165 (2907359)	2907359..2908363	+	1005	WP_004179822.1	fimbrial protein	-
KJA60_RS14170 (2908505)	2908505..2909797	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
KJA60_RS14175 (2909814)	2909814..2911136	-	1323	WP_004179815.1	type II toxin-antitoxin system HipA family toxin	Toxin
KJA60_RS14180 (2911136)	2911136..2911402	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KJA60_RS14185 (2911668)	2911668..2911994	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
KJA60_RS14190 (2911991)	2911991..2912491	-	501	WP_004179811.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
KJA60_RS14195 (2912469)	2912469..2913848	-	1380	WP_094932908.1	nucleobase:cation symporter-2 family protein	-
KJA60_RS14200 (2914194)	2914194..2915348	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
KJA60_RS14205 (2915329)	2915329..2916252	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	2704042..2946237	242195

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49152.42 Da
• Isoelectric Point: 7.2850

>T203439 WP_004179815.1 NZ_CP075316:c2911136-2909814 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTHQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T203439 NZ_CP100087:1427558-1427968 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAAATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT203439 WP_004176198.1 NZ_CP075316:c2911402-2911136 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT203439 NZ_CP100087:1427311-1427577 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA