Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 843035..843945 Replicon chromosome
Accession NZ_CP075316
Organism Klebsiella pneumoniae strain DD521

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag KJA60_RS04210 Protein ID WP_004026354.1
Coordinates 843035..843505 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag KJA60_RS04215 Protein ID WP_004181895.1
Coordinates 843502..843945 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJA60_RS04190 (839368) 839368..840126 + 759 Protein_829 putrescine ABC transporter permease SapB -
KJA60_RS04195 (840169) 840169..840873 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
KJA60_RS04200 (840898) 840898..842094 + 1197 WP_119062602.1 IS481 family transposase -
KJA60_RS04205 (842665) 842665..842925 + 261 WP_004026352.1 hypothetical protein -
KJA60_RS04210 (843035) 843035..843505 - 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
KJA60_RS04215 (843502) 843502..843945 - 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
KJA60_RS04220 (844046) 844046..845317 - 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
KJA60_RS04225 (845317) 845317..845739 - 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
KJA60_RS04230 (845825) 845825..847312 - 1488 WP_004178082.1 group II intron reverse transcriptase/maturase -
KJA60_RS04235 (847825) 847825..848079 + 255 WP_016946352.1 Hha/YmoA family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T203428 WP_004026354.1 NZ_CP075316:c843505-843035 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T203428 NZ_CP100084:c5361773-5361390 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT203428 WP_004181895.1 NZ_CP075316:c843945-843502 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT203428 NZ_CP100084:c5362015-5361773 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References