Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 56129..56865 Replicon plasmid pYTF44-1-233k
Accession NZ_CP075288
Organism Klebsiella pneumoniae strain YTF44-1

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag KI725_RS27540 Protein ID WP_003026803.1
Coordinates 56383..56865 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KI725_RS27535 Protein ID WP_003026799.1
Coordinates 56129..56395 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI725_RS27490 (KI725_27490) 52191..52553 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
KI725_RS27495 (KI725_27495) 52603..52953 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
KI725_RS27500 (KI725_27500) 53311..53580 + 270 WP_004152102.1 hypothetical protein -
KI725_RS27505 (KI725_27505) 53568..54143 + 576 WP_004152103.1 hypothetical protein -
KI725_RS27510 (KI725_27510) 54174..54668 + 495 WP_004152104.1 DNA-binding protein -
KI725_RS27515 (KI725_27515) 54712..55080 + 369 WP_004152105.1 hypothetical protein -
KI725_RS27520 (KI725_27520) 55114..55317 + 204 WP_004152106.1 HHA domain-containing protein -
KI725_RS27525 (KI725_27525) 55366..55623 + 258 WP_004152107.1 hypothetical protein -
KI725_RS27530 (KI725_27530) 55699..55953 + 255 WP_004152108.1 hypothetical protein -
KI725_RS27535 (KI725_27535) 56129..56395 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KI725_RS27540 (KI725_27540) 56383..56865 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
KI725_RS27545 (KI725_27545) 57066..58469 + 1404 WP_001272054.1 ISNCY-like element ISKpn21 family transposase -
KI725_RS27550 (KI725_27550) 58498..59130 - 633 WP_001567369.1 hypothetical protein -
KI725_RS27555 (KI725_27555) 59319..60608 + 1290 Protein_63 ISNCY family transposase -
KI725_RS27560 (KI725_27560) 60628..61836 - 1209 WP_001339197.1 IS4-like element ISVsa5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid floR / dfrA12 / aadA2 / qacE / sul1 / mph(A) / aph(3')-Ia - 1..233263 233263


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T203424 WP_003026803.1 NZ_CP075288:56383-56865 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T203424 NZ_CP100084:4148226-4148444 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT203424 WP_003026799.1 NZ_CP075288:56129-56395 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT203424 NZ_CP100084:4147825-4148199 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References