Detailed information of TA system TA203423

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 65813..66456
• Replicon: plasmid pYTF44-1-tmexCD
• Accession: NZ_CP075287
• Organism: Klebsiella pneumoniae strain YTF44-1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q84A06
• Locus tag: KI725_RS27150
• Protein ID: WP_000754566.1
• Coordinates: 65813..66229 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: KI725_RS27155
• Protein ID: WP_001261276.1
• Coordinates: 66226..66456 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI725_RS27130 (KI725_27130)	61269..61793	-	525	Protein_67	SDR family oxidoreductase	-
KI725_RS28685	63274..63375	+	102	Protein_69	Tn3 family transposase	-
KI725_RS27140 (KI725_27140)	63395..64471	-	1077	WP_000227969.1	IS110 family transposase	-
KI725_RS27145 (KI725_27145)	64753..65609	-	857	Protein_71	IS3-like element ISEc15 family transposase	-
KI725_RS27150 (KI725_27150)	65813..66229	-	417	WP_000754566.1	type II toxin-antitoxin system VapC family toxin	Toxin
KI725_RS27155 (KI725_27155)	66226..66456	-	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KI725_RS27160 (KI725_27160)	67030..67380	+	351	WP_000493378.1	hypothetical protein	-
KI725_RS27165 (KI725_27165)	67431..68174	+	744	WP_000129823.1	hypothetical protein	-
KI725_RS27170 (KI725_27170)	68171..68947	+	777	WP_000015958.1	site-specific integrase	-
KI725_RS27175 (KI725_27175)	69005..69262	-	258	WP_000764642.1	hypothetical protein	-
KI725_RS27180 (KI725_27180)	69391..69495	-	105	WP_032409716.1	hypothetical protein	-
KI725_RS27185 (KI725_27185)	70025..70891	+	867	WP_004118283.1	replication initiation protein	-
KI725_RS27190 (KI725_27190)	71068..71337	-	270	WP_000339857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(A) / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aac(3)-IVa / aph(4)-Ia / aph(6)-Id / aph(3'')-Ib / aph(3')-Ia / mph(E) / msr(E) / armA / sul1 / blaDHA-1 / qnrB4	-	1..84019	84019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15006.52 Da
• Isoelectric Point: 9.2957

>T203423 WP_000754566.1 NZ_CP075287:c66229-65813 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T203423 NZ_CP100084:c4023188-4022871 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT203423 WP_001261276.1 NZ_CP075287:c66456-66226 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT203423 NZ_CP100084:c4022878-4022591 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: A0A387K1G3

Antitoxin

• AlphaFold DB: A0A387K023