Detailed information of TA system TA020341

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 5333913..5334508
• Replicon: chromosome
• Accession: NC_002655
• Organism: Escherichia coli O157:H7 str. EDL933

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: Z_RS27330
• Protein ID: WP_000239579.1
• Coordinates: 5334158..5334508 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: Z_RS27325
• Protein ID: WP_001223210.1
• Coordinates: 5333913..5334164 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Z_RS27315 (Z5832)	5329578..5333357	+	3780	WP_000060956.1	autotransporter assembly complex protein TamB	-
Z_RS27320 (Z5833)	5333360..5333701	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
Z_RS27325 (Z5835)	5333913..5334164	+	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
Z_RS27330 (Z5836)	5334158..5334508	+	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
Z_RS27335 (Z5837)	5334588..5335118	-	531	WP_000055075.1	inorganic diphosphatase	-
Z_RS27340 (Z5838)	5335428..5336384	+	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
Z_RS27345 (Z5839)	5336524..5338026	+	1503	WP_000205806.1	sugar ABC transporter ATP-binding protein	-
Z_RS27350 (Z5840)	5338040..5339062	+	1023	WP_001301928.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T20341 WP_000239579.1 NC_002655:5334158-5334508 [Escherichia coli O157:H7 str. EDL933]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T20341 NC_002655:5334158-5334508 [Escherichia coli O157:H7 str. EDL933]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT20341 WP_001223210.1 NC_002655:5333913-5334164 [Escherichia coli O157:H7 str. EDL933]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT20341 NC_002655:5333913-5334164 [Escherichia coli O157:H7 str. EDL933]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8