Detailed information of TA system TA203404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 121666..122188
• Replicon: plasmid pSZP4-9-2-151k
• Accession: NZ_CP075261
• Organism: Klebsiella pneumoniae strain SZP4-9-2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D0QMR1
• Locus tag: KI741_RS28980
• Protein ID: WP_000220561.1
• Coordinates: 121666..121947 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: KI741_RS28985
• Protein ID: WP_000121743.1
• Coordinates: 121937..122188 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI741_RS28940 (117242)	117242..117466	-	225	WP_000743213.1	hypothetical protein	-
KI741_RS28945 (117463)	117463..118200	-	738	WP_001446887.1	recombinase family protein	-
KI741_RS28950 (118686)	118686..118826	-	141	WP_001044210.1	hypothetical protein	-
KI741_RS28955 (118832)	118832..119536	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
KI741_RS28960 (119669)	119669..119911	-	243	WP_000008708.1	hypothetical protein	-
KI741_RS28965 (119901)	119901..120170	-	270	WP_001006718.1	hypothetical protein	-
KI741_RS28970 (120179)	120179..120727	-	549	WP_001178641.1	hypothetical protein	-
KI741_RS28975 (120801)	120801..121316	-	516	WP_001025387.1	J domain-containing protein	-
KI741_RS28980 (121666)	121666..121947	-	282	WP_000220561.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KI741_RS28985 (121937)	121937..122188	-	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
KI741_RS28990 (123423)	123423..124262	+	840	WP_001352874.1	replication initiation protein	-
KI741_RS28995 (124283)	124283..125125	+	843	WP_000435064.1	replication initiation protein	-
KI741_RS29000 (125129)	125129..125575	+	447	WP_001074384.1	hypothetical protein	-
KI741_RS29005 (125712)	125712..126068	+	357	WP_001714234.1	DNA distortion polypeptide 3	-
KI741_RS29010 (126089)	126089..126949	-	861	WP_063122221.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / qnrS1 / blaCTX-M-3 / blaTEM-1B / floR / tet(A) / sul1	-	1..151651	151651

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11023.86 Da
• Isoelectric Point: 10.5938

>T203404 WP_000220561.1 NZ_CP075261:c121947-121666 [Klebsiella pneumoniae]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Nucleotide

• Length: 282 bp

>T203404 NZ_CP100076:269837-270205 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT203404 WP_000121743.1 NZ_CP075261:c122188-121937 [Klebsiella pneumoniae]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT203404 NZ_CP100076:269619-269840 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A3S5I302

Antitoxin

• AlphaFold DB: A0A3S5I301