Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 23404..23929 Replicon plasmid pSZP4-9-2-151k
Accession NZ_CP075261
Organism Klebsiella pneumoniae strain SZP4-9-2

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag KI741_RS28375 Protein ID WP_013023785.1
Coordinates 23624..23929 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag KI741_RS28370 Protein ID WP_001568025.1
Coordinates 23404..23622 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI741_RS28345 (18560) 18560..19339 + 780 WP_013023780.1 hypothetical protein -
KI741_RS28350 (19615) 19615..21138 + 1524 WP_017899887.1 hypothetical protein -
KI741_RS28355 (21172) 21172..22299 + 1128 WP_013023782.1 DUF4238 domain-containing protein -
KI741_RS28360 (22296) 22296..22586 + 291 WP_013023783.1 hypothetical protein -
KI741_RS28365 (22576) 22576..22773 - 198 WP_017899886.1 hypothetical protein -
KI741_RS28370 (23404) 23404..23622 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KI741_RS28375 (23624) 23624..23929 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
KI741_RS28380 (24098) 24098..24493 + 396 WP_017899885.1 hypothetical protein -
KI741_RS28385 (24520) 24520..24834 + 315 WP_053389906.1 hypothetical protein -
KI741_RS28390 (24845) 24845..25861 + 1017 WP_017899884.1 hypothetical protein -
KI741_RS28395 (26059) 26059..26853 + 795 WP_004197635.1 site-specific integrase -
KI741_RS29410 (27317) 27317..27619 - 303 WP_004197636.1 hypothetical protein -
KI741_RS28405 (27616) 27616..28242 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / qnrS1 / blaCTX-M-3 / blaTEM-1B / floR / tet(A) / sul1 - 1..151651 151651


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T203403 WP_013023785.1 NZ_CP075261:23624-23929 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T203403 NZ_CP100075:755-1021 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT203403 WP_001568025.1 NZ_CP075261:23404-23622 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT203403 NZ_CP100075:547-771 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References