Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 36216..37126 Replicon plasmid pSZP4-9-2-tmexCD
Accession NZ_CP075257
Organism Klebsiella pneumoniae strain SZP4-9-2

Toxin (Protein)


Gene name - Uniprot ID A0A5Q9LR54
Locus tag KI741_RS26420 Protein ID WP_004181896.1
Coordinates 36656..37126 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag KI741_RS26415 Protein ID WP_004181895.1
Coordinates 36216..36659 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI741_RS26390 (KI741_26395) 31371..31586 - 216 Protein_43 IS200/IS605 family transposase -
KI741_RS26395 (KI741_26400) 31588..32157 + 570 Protein_44 transposase -
KI741_RS26400 (KI741_26405) 32849..34336 + 1488 WP_004178082.1 group II intron reverse transcriptase/maturase -
KI741_RS26405 (KI741_26410) 34422..34844 + 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
KI741_RS26410 (KI741_26415) 34844..36115 + 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
KI741_RS26415 (KI741_26420) 36216..36659 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
KI741_RS26420 (KI741_26425) 36656..37126 + 471 WP_004181896.1 RES family NAD+ phosphorylase Toxin
KI741_RS26425 (KI741_26430) 37236..37496 - 261 WP_004026352.1 hypothetical protein -
KI741_RS26430 (KI741_26435) 38195..39564 + 1370 WP_085949440.1 IS3-like element ISKpn8 family transposase -
KI741_RS26435 (KI741_26440) 39561..40694 - 1134 WP_171888422.1 IS481-like element ISKpn28 family transposase -
KI741_RS26440 (KI741_26445) 40766..40924 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul3 / aac(3)-IVa / aph(4)-Ia / aph(6)-Id / aph(3'')-Ib / aph(3')-Ia / mph(E) / msr(E) / armA / sul1 / blaDHA-1 / qnrB4 - 1..274231 274231
- flank IS/Tn - - 31771..32157 386


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17549.81 Da        Isoelectric Point: 4.6155

>T203402 WP_004181896.1 NZ_CP075257:36656-37126 [Klebsiella pneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T203402 NZ_CP100073:c98572-98423 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT203402 WP_004181895.1 NZ_CP075257:36216-36659 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT203402 NZ_CP100073:98617-98676 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LR54

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References