Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3976862..3977598 Replicon chromosome
Accession NZ_CP075256
Organism Klebsiella pneumoniae strain SZP4-9-2

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag KI741_RS19320 Protein ID WP_032433360.1
Coordinates 3976862..3977344 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KI741_RS19325 Protein ID WP_003026799.1
Coordinates 3977332..3977598 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI741_RS19295 (3972168) 3972168..3972746 + 579 WP_032432061.1 type 1 fimbrial protein -
KI741_RS19300 (3972758) 3972758..3973702 + 945 WP_077254249.1 fimbrial protein -
KI741_RS19305 (3973915) 3973915..3974514 + 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
KI741_RS19310 (3974865) 3974865..3975260 - 396 WP_004143398.1 helix-turn-helix domain-containing protein -
KI741_RS19315 (3975309) 3975309..3976655 - 1347 WP_077253535.1 ISNCY family transposase -
KI741_RS19320 (3976862) 3976862..3977344 - 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
KI741_RS19325 (3977332) 3977332..3977598 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KI741_RS19330 (3977911) 3977911..3978474 - 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
KI741_RS19335 (3978484) 3978484..3979116 - 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
KI741_RS19340 (3979134) 3979134..3980138 - 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
KI741_RS19345 (3980122) 3980122..3980784 - 663 WP_032433366.1 dihydroxyacetone kinase subunit DhaL -
KI741_RS19350 (3980813) 3980813..3981952 - 1140 WP_032433368.1 mannitol dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3962671..4009653 46982
- flank IS/Tn - - 3975309..3976667 1358


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T203397 WP_032433360.1 NZ_CP075256:c3977344-3976862 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T203397 NZ_CP100072:c4848294-4848010 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT203397 WP_003026799.1 NZ_CP075256:c3977598-3977332 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT203397 NZ_CP100072:c4848526-4848284 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References