Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 161769..162394 Replicon chromosome
Accession NZ_CP075256
Organism Klebsiella pneumoniae strain SZP4-9-2

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KI741_RS00805 Protein ID WP_002882817.1
Coordinates 162011..162394 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KI741_RS00800 Protein ID WP_004150355.1
Coordinates 161769..162011 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI741_RS00775 (157760) 157760..158359 + 600 WP_004151865.1 glucose-1-phosphatase -
KI741_RS00780 (158353) 158353..159213 + 861 WP_004146232.1 virulence factor BrkB family protein -
KI741_RS00785 (159210) 159210..159647 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KI741_RS00790 (159692) 159692..160633 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KI741_RS00795 (160647) 160647..161564 - 918 WP_021466676.1 alpha/beta hydrolase -
KI741_RS00800 (161769) 161769..162011 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KI741_RS00805 (162011) 162011..162394 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KI741_RS00810 (162568) 162568..163497 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KI741_RS00815 (163494) 163494..164129 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KI741_RS00820 (164126) 164126..165028 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T203383 WP_002882817.1 NZ_CP075256:162011-162394 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T203383 NZ_CP099976:c15227-14955 [Halomonas sp. Y3]
ATGCTGACCCCGGTCCGAACAAGCCAGTTCAAACGAGACGTGAAGCGGGTCCAGAAGCGCGGCAAGGACATGACCAAGCT
GCGGACCCTGCTTGGGCTGCTAATTGAGCAGGCCCCTTTACCCGAGGCCTACCAGGACCACCCCTTGAGAGGGAACTGGA
ACGGCTATCGGGATGCGCATATTGAGCCCGACTGGCTACTTCTGTATCGCATCGTGGATGATGAACTGCACCTGTCCCGA
ACGGGCAGTCATGCCGACTTGTTCCGTGAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT203383 WP_004150355.1 NZ_CP075256:161769-162011 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT203383 NZ_CP099976:c15484-15227 [Halomonas sp. Y3]
ATGAGTGCTGATACCGTGGTGAGAGCCCGCATCGACAGCGAGACCAAAGAGCGTGCCACCGCCGCCCTGGAGGCCATGGG
GCTGTCCGTGTCTGATGCCATTCGCTTGCTGATGCTGCGCATTGCAGACGAGAAGCGCCTGCCGTTTGCGGTGCAAGTGC
CGAACGCCACCACCACCAAGGCTATGGCCGAGCTGGACGCCGGCAAGGGGAAGCGGTTCGACAACGCCGATGAGCTGTTT
CAGGACCTGGGGATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References