Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4426674..4427305 Replicon chromosome
Accession NC_002655
Organism Escherichia coli O157:H7 str. EDL933

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag Z_RS22935 Protein ID WP_001259386.1
Coordinates 4427030..4427305 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag Z_RS22930 Protein ID WP_000593555.1
Coordinates 4426674..4427033 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Z_RS22900 (Z4875) 4421785..4422258 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
Z_RS22905 (Z4876) 4422255..4422536 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
Z_RS22910 (Z4877) 4422613..4423971 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
Z_RS22915 (Z4878) 4423964..4425472 + 1509 WP_001301773.1 carbohydrate kinase -
Z_RS22920 (Z4879) 4425462..4425731 + 270 WP_000933062.1 HPr family phosphocarrier protein -
Z_RS22925 (Z4881) 4425764..4426624 + 861 WP_001131788.1 class II aldolase -
Z_RS22930 (Z4882) 4426674..4427033 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
Z_RS22935 (Z4883) 4427030..4427305 - 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
Z_RS22940 (Z4884) 4427378..4428502 - 1125 WP_001301659.1 ABC transporter permease -
Z_RS22945 (Z4885) 4428502..4431237 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
Z_RS22950 (Z4886) 4431234..4432301 - 1068 WP_000361459.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T20337 WP_001259386.1 NC_002655:c4427305-4427030 [Escherichia coli O157:H7 str. EDL933]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T20337 NC_002655:c4427305-4427030 [Escherichia coli O157:H7 str. EDL933]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT20337 WP_000593555.1 NC_002655:c4427033-4426674 [Escherichia coli O157:H7 str. EDL933]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT20337 NC_002655:c4427033-4426674 [Escherichia coli O157:H7 str. EDL933]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References