Detailed information of TA system TA203369

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 88723..90449
• Replicon: chromosome
• Accession: NZ_CP075191
• Organism: Ferribacterium limneticum strain 128

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: KI617_RS00395
• Protein ID: WP_226449611.1
• Coordinates: 88723..89367 (+)
• Length: 215 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: KI617_RS00400
• Protein ID: WP_226449613.1
• Coordinates: 89367..90449 (+)
• Length: 361 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI617_RS20310 (KI617_00380)	84137..85282	+	1146	WP_264180038.1	class I SAM-dependent DNA methyltransferase	-
KI617_RS20315 (KI617_00385)	85230..86909	+	1680	WP_264180039.1	SAM-dependent methyltransferase	-
KI617_RS00385 (KI617_00390)	86902..88149	+	1248	WP_226449607.1	restriction endonuclease subunit S	-
KI617_RS00390 (KI617_00395)	88154..88726	+	573	WP_226449609.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
KI617_RS00395 (KI617_00400)	88723..89367	+	645	WP_226449611.1	DUF4433 domain-containing protein	Toxin
KI617_RS00400 (KI617_00405)	89367..90449	+	1083	WP_226449613.1	macro domain-containing protein	Antitoxin
KI617_RS00405 (KI617_00410)	90458..93718	+	3261	WP_226449615.1	HsdR family type I site-specific deoxyribonuclease	-
KI617_RS00410 (KI617_00415)	93718..94425	+	708	WP_226449617.1	SprT family zinc-dependent metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (14-214)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 215 a.a.
• Molecular weight: 24644.22 Da
• Isoelectric Point: 7.9511

>T203369 WP_226449611.1 NZ_CP075191:88723-89367 [Ferribacterium limneticum]
MIYKNLNPEKALIWRIVHRDNLPWILDNGLHCRNSQFQDPNFVNIGNPELIDKRAHRGVPITPGGTLSDYVPFYFTPFSV
MMKNIHSGWGGIRQRSNAEIVILVSSLRRVQEQGIPFLFTNGHAYPPDTRFFNDLSDLSVIDWGILQKRDFKRDLDDPKK
LERYQAEALIHQHLPVSGLVGAVCYTEAIKSDIEQQILARGLSLQVVARTEWYF

Nucleotide

• Length: 645 bp

>T203369 NZ_CP099973:c4489254-4488943 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 361 a.a.
• Molecular weight: 40541.90 Da
• Isoelectric Point: 6.8696

>AT203369 WP_226449613.1 NZ_CP075191:89367-90449 [Ferribacterium limneticum]
MIRFTQGNLLESQAEALVNTVNTVGVMGKGIALMFKERFSANFLAYAAACKAKQVQTGKMFVTEVHELGGPRWIVNFPTK
RHWKAPSELVWVKDGLQDLRRFLLEKKVKSIAIPPLGAGNGGLEWPEVREQIKLALEDLDVEILVFEPTSQYQNVAKRSG
TEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFMERAIERFSLENNPLKLEFKAHIYGPFTPKLNFVLDNLDGSYLH
CDKRISDASPLDVIWFDDSRKDLVQAYLKTEARQYSEALEYVGELIDGFESPFGMELLATVDWLLTRENAEPTVTGIRQA
LGAWPAGAAAAARKLRLFDERSVGFALERLTKAQPVPLAA

Nucleotide

• Length: 1083 bp

>AT203369 NZ_CP099973:c4488942-4488652 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA