Detailed information of TA system TA203366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2177019..2177671
• Replicon: chromosome
• Accession: NZ_CP075189
• Organism: Ferribacterium limneticum strain 77

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KI610_RS10470
• Protein ID: WP_226494922.1
• Coordinates: 2177270..2177671 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KI610_RS10465
• Protein ID: WP_226494921.1
• Coordinates: 2177019..2177258 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI610_RS10440 (KI610_10400)	2172339..2173454	+	1116	WP_226494916.1	Fic family protein	-
KI610_RS10445 (KI610_10405)	2173547..2174605	+	1059	WP_226494917.1	dTDP-glucose 4,6-dehydratase	-
KI610_RS10450 (KI610_10410)	2174608..2175495	+	888	WP_226494918.1	dTDP-4-dehydrorhamnose reductase	-
KI610_RS10455 (KI610_10415)	2175510..2176388	+	879	WP_226494919.1	glucose-1-phosphate thymidylyltransferase RfbA	-
KI610_RS10460 (KI610_10420)	2176388..2176930	+	543	WP_226494920.1	dTDP-4-dehydrorhamnose 3,5-epimerase	-
KI610_RS10465 (KI610_10425)	2177019..2177258	+	240	WP_226494921.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KI610_RS10470 (KI610_10430)	2177270..2177671	+	402	WP_226494922.1	type II toxin-antitoxin system VapC family toxin	Toxin
KI610_RS10475 (KI610_10435)	2177768..2179207	+	1440	WP_226494923.1	mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase	-
KI610_RS10480 (KI610_10440)	2179311..2179847	+	537	WP_226494924.1	FxDxF family PEP-CTERM protein	-
KI610_RS10485 (KI610_10445)	2179914..2181017	+	1104	WP_226494925.1	NDP-sugar synthase	-
KI610_RS10490 (KI610_10450)	2181031..2181390	+	360	WP_226494926.1	VanZ family protein	-
KI610_RS10495 (KI610_10455)	2181406..2182209	-	804	WP_226494927.1	LuxR C-terminal-related transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2157716..2177671	19955

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-42)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14358.55 Da
• Isoelectric Point: 6.2206

>T203366 WP_226494922.1 NZ_CP075189:2177270-2177671 [Ferribacterium limneticum]
MRFMLDTNICIYAIKNRPAEVLQALRAHEAAGIGVSSITVAELFFGVEKSGSGKNRLALQHFLEPLEIADFDVAAAQVYG
AVRQQLELAGTPIGPLDTQIAAHALSLGVTLVSNNLREFSRVKALQVENWVGV

Nucleotide

• Length: 402 bp

>T203366 NZ_CP099973:c4031804-4031496 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGATTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGACGGGTTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8983.07 Da
• Isoelectric Point: 4.8279

>AT203366 WP_226494921.1 NZ_CP075189:2177019-2177258 [Ferribacterium limneticum]
MQTAKLFQNGRSQAVRLPKAFRFEGVSEVRIERDGDRVVLSPASRPSIEGLIAALDEFDRENPMPEREQVAAPDQRESW

Nucleotide

• Length: 240 bp

>AT203366 NZ_CP099973:c4032046-4031807 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTGGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCAGCGGTGCTTACCGCCGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA