Detailed information of TA system TA203353

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 1864745..1866249
• Replicon: chromosome
• Accession: NZ_CP075176
• Organism: Pseudomonas aeruginosa strain PA790

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9HYX1
• Locus tag: KIK44_RS08660
• Protein ID: WP_003119682.1
• Coordinates: 1864745..1865332 (+)
• Length: 196 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A9E8LAI1
• Locus tag: KIK44_RS08665
• Protein ID: WP_003117606.1
• Coordinates: 1865395..1866249 (+)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIK44_RS08655 (KIK44_08655)	1861000..1864479	-	3480	WP_033938798.1	PAS domain-containing hybrid sensor histidine kinase/response regulator	-
KIK44_RS08660 (KIK44_08660)	1864745..1865332	+	588	WP_003119682.1	GNAT family protein	Toxin
KIK44_RS08665 (KIK44_08665)	1865395..1866249	+	855	WP_003117606.1	AraC family transcriptional regulator	Antitoxin
KIK44_RS08670 (KIK44_08670)	1866346..1868511	+	2166	WP_033989266.1	TonB-dependent receptor family protein	-
KIK44_RS08675 (KIK44_08675)	1868686..1870560	+	1875	WP_003117607.1	MFS transporter	-
KIK44_RS08680 (KIK44_08680)	1870621..1870830	-	210	WP_003091604.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (20-152)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (19-151)

Sequences

Toxin

Protein

• Length: 196 a.a.
• Molecular weight: 21901.23 Da
• Isoelectric Point: 9.8676

>T203353 WP_003119682.1 NZ_CP075176:1864745-1865332 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAALEASFTG

Nucleotide

• Length: 588 bp

>T203353 NZ_CP099968:c1236047-1235742 [Brucella pseudintermedia]
ATGAAGAAGATTGAAGTGGCTGCATGGGACAGCGCGGAAGTTCTCGACACGCCCGAGGCCATTGAAGAATACCTTGCCGC
CGCATTTGAGACGGAGGACCCGGTGTTCATCGCCAAGGCGCTCGGCGTCGTTGCCCGCGCTCGCAATATGAGCAAATTGG
CTCGCAATACAGGAATGACACGGGCGGCGCTTTATCGCGCTTTCAACGGCGAGACGAGTCCGGAGTTCGCGACGATCATG
AAGATCATGCATGCGCTAGGCGTCAGCATTACTCCCGTGGTGAAAAAGACCGAGGAAGCGGCTTGA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31863.14 Da
• Isoelectric Point: 7.5295

>AT203353 WP_003117606.1 NZ_CP075176:1865395-1866249 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHAGSLETQTRWRETVLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTTYE

Nucleotide

• Length: 855 bp

>AT203353 NZ_CP099968:c1236339-1236034 [Brucella pseudintermedia]
ATGCTTACGATCAAACGTACCACTATCTTTATTGATTGGCTCAAGGGTCTGAAAGACCGTCGTGCCGTCGCGCGGATTGC
GATGAGAATTGACAGATTTGAGTCTGGCAACCCCGGTCATGTACGAGATGTTGGCGATGGCGTGGGCGAGATGAAAATCG
ACTATGGGCCGGGCTACCGAGTCTATTATGTCCGCCGCGAAAAGACCATTTATCTATTACTTTGCGGTGGAGACAAGCGA
ACGCAGGACAGAGATATAGCCGAAGCCAAGCGCATCGCAAAGGAGTGGACAGATGAAGAAGATTGA

Structures

Toxin

• PDB: 1YRE
• PDB: 8GXJ