Detailed information of TA system TA203351

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 158041..158546
• Replicon: chromosome
• Accession: NZ_CP075176
• Organism: Pseudomonas aeruginosa strain PA790

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: KIK44_RS00725
• Protein ID: WP_003083773.1
• Coordinates: 158041..158322 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: KIK44_RS00730
• Protein ID: WP_003083775.1
• Coordinates: 158319..158546 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIK44_RS00700 (KIK44_00700)	153292..154641	+	1350	WP_003119513.1	C4-dicarboxylate transporter DctA	-
KIK44_RS00705 (KIK44_00705)	154690..155376	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
KIK44_RS00710 (KIK44_00710)	155477..156211	+	735	WP_003110658.1	GntR family transcriptional regulator	-
KIK44_RS00715 (KIK44_00715)	156391..156801	+	411	WP_003110659.1	aegerolysin family protein	-
KIK44_RS00720 (KIK44_00720)	156833..157741	-	909	WP_003083769.1	LysR family transcriptional regulator	-
KIK44_RS00725 (KIK44_00725)	158041..158322	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
KIK44_RS00730 (KIK44_00730)	158319..158546	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
KIK44_RS00735 (KIK44_00735)	158722..159342	-	621	WP_003101226.1	hypothetical protein	-
KIK44_RS00740 (KIK44_00740)	159443..159943	+	501	WP_003162763.1	LEA type 2 family protein	-
KIK44_RS00745 (KIK44_00745)	160016..160357	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
KIK44_RS00750 (KIK44_00750)	160439..161866	-	1428	WP_003083784.1	GABA permease	-
KIK44_RS00755 (KIK44_00755)	162035..163528	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T203351 WP_003083773.1 NZ_CP075176:c158322-158041 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T203351 NZ_CP099967:c1290037-1289639 [Brucella pseudintermedia]
ATGCGCTATATGTTAGACACCAACATCATCAGCGATCTGGTAAAAAATCCGGCGGGTTCGGTTGCCGGACATATCAGGCG
GGTTGGGGCAGATGCCGTTTGCACCAGCATCATCGTTGCTGCCGAACTTCGTTATGGTGTTCTGAAAAAAGGTTCGCCTG
CCCTGGCCGAGCGTGTCGAGGCCATTCTTCGGGAGATACCTGTTCTGCCGTTCGATGTTCCCGCCGATGCAAAATATGGA
GCGCTTCGGGCTGCGCTTGAAGCAAAAGGGCAGCCGATTGGCGGCAATAACCTTCTGATTGCAGCCCATGCCGCCACGCT
TGGAAAAACCATGGTGACGGCCAATGTGTCCGAGTTCGGACGGGTCAGCGAATTATCGGTCGAAAACTGGCTCGAATAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT203351 WP_003083775.1 NZ_CP075176:c158546-158319 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT203351 NZ_CP099967:c1290288-1290037 [Brucella pseudintermedia]
ATGTCCCAGAAACCTCGGCCCAAATCGCGGGAAGTCCGGCTGTTCCGCAACAATCGCAATCAGGCAATCCGGATTCCGGT
CGAATTCGAGCTGCCGGGCGATCGGGCATTGATTACGCGCGACGGCGACCGGCTGATCGTCGAACCGCTTCGCCGGGGCG
GACTGCTGGCCCTGCTCGACAGCTGGAAACCCCTGGATGAAGCCCTGCCTGATGTTGCGGACCGCCCGGTCGAGCCGAAA
GACATCTTCTGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9