Detailed information of TA system TA020334

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 4335187..4335941
• Replicon: chromosome
• Accession: NC_002655
• Organism: Escherichia coli O157:H7 str. EDL933

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: Z_RS22470
• Protein ID: WP_001301452.1
• Coordinates: 4335456..4335941 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: Q8X700
• Locus tag: Z_RS22465
• Protein ID: WP_000801912.1
• Coordinates: 4335187..4335465 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Z_RS22460 (Z4774)	4332415..4335120	+	2706	WP_000906970.1	HTH-type transcriptional regulator MalT	-
Z_RS22465 (Z4776)	4335187..4335465	+	279	WP_000801912.1	DUF1778 domain-containing protein	Antitoxin
Z_RS22470 (Z4777)	4335456..4335941	+	486	WP_001301452.1	GNAT family N-acetyltransferase	Toxin
Z_RS22475 (Z4778)	4335991..4337019	-	1029	WP_010904929.1	RNA 3'-terminal phosphate cyclase	-
Z_RS22480 (Z4779)	4337023..4338249	-	1227	WP_001105473.1	RNA-splicing ligase RtcB	-
Z_RS22485 (Z4780)	4338437..4340035	+	1599	WP_001232986.1	DNA-binding transcriptional regulator RtcR	-
Z_RS22490 (Z4781)	4340017..4340775	-	759	WP_001302007.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17650.57 Da
• Isoelectric Point: 9.4066

>T20334 WP_001301452.1 NC_002655:4335456-4335941 [Escherichia coli O157:H7 str. EDL933]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E

Nucleotide

• Length: 486 bp

>T20334 NC_002655:4335456-4335941 [Escherichia coli O157:H7 str. EDL933]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10420.94 Da
• Isoelectric Point: 4.6336

>AT20334 WP_000801912.1 NC_002655:4335187-4335465 [Escherichia coli O157:H7 str. EDL933]
MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFILDDEQYDAFLAALDAPITAERQAK
INALMNRKSPWE

Nucleotide

• Length: 279 bp

>AT20334 NC_002655:4335187-4335465 [Escherichia coli O157:H7 str. EDL933]
ATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTCGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LHB9