Detailed information of TA system TA203334

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1835763..1836353
• Replicon: chromosome
• Accession: NZ_CP075174
• Organism: Salmonella enterica subsp. houtenae serovar 45:gz51:- strain 20-369

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: V0VE57
• Locus tag: KI721_RS08705
• Protein ID: WP_000373724.1
• Coordinates: 1836021..1836353 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: KI721_RS08700
• Protein ID: WP_079812695.1
• Coordinates: 1835763..1836020 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI721_RS08675 (1832103)	1832103..1833569	-	1467	WP_079812684.1	C69 family dipeptidase	-
KI721_RS08680 (1834063)	1834063..1834269	+	207	WP_000661532.1	hypothetical protein	-
KI721_RS08685 (1834463)	1834463..1834729	-	267	WP_125873103.1	bacteriocin	-
KI721_RS08690 (1835018)	1835018..1835211	+	194	Protein_1698	hypothetical protein	-
KI721_RS08695 (1835208)	1835208..1835415	+	208	Protein_1699	helix-turn-helix domain-containing protein	-
KI721_RS08700 (1835763)	1835763..1836020	+	258	WP_079812695.1	antitoxin	Antitoxin
KI721_RS08705 (1836021)	1836021..1836353	+	333	WP_000373724.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KI721_RS08710 (1837002)	1837002..1837877	+	876	Protein_1702	integrase domain-containing protein	-
KI721_RS08715 (1838404)	1838404..1839653	-	1250	Protein_1703	tyrosine-type recombinase/integrase	-
KI721_RS08725 (1840246)	1840246..1841345	+	1100	WP_134953911.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11724.57 Da
• Isoelectric Point: 10.5834

>T203334 WP_000373724.1 NZ_CP075174:1836021-1836353 [Salmonella enterica subsp. houtenae serovar 45:g,z51:-]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARSGKRLERIPDAVVNEVLARLEAILS

Nucleotide

• Length: 333 bp

>T203334 NZ_CP099914:c608220-607756 [Escherichia albertii]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAACCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTTTCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACACTGCGCACCTACGGTAAAAAAACAGATGCCTATACAGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCAAGAGACAGAAGAAAAGCATTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9323.75 Da
• Isoelectric Point: 4.4341

>AT203334 WP_079812695.1 NZ_CP075174:1835763-1836020 [Salmonella enterica subsp. houtenae serovar 45:g,z51:-]
MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSDVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT203334 NZ_CP099914:c608555-608220 [Escherichia albertii]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCTGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0VE57