Detailed information of TA system TA203331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 4093511..4094184
• Replicon: chromosome
• Accession: NZ_CP075171
• Organism: Leptodesmis sichuanensis PKUAC-SCTA121

Toxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KIK02_RS19125
• Protein ID: WP_233744141.1
• Coordinates: 4093762..4094184 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KIK02_RS19120
• Protein ID: WP_233744140.1
• Coordinates: 4093511..4093765 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIK02_RS19100 (KIK02_19240)	4089636..4090169	-	534	WP_233744137.1	Uma2 family endonuclease	-
KIK02_RS19105 (KIK02_19245)	4090653..4091712	-	1060	Protein_3805	ISKra4 family transposase	-
KIK02_RS19110 (KIK02_19255)	4092093..4092728	-	636	WP_233744138.1	Uma2 family endonuclease	-
KIK02_RS19115 (KIK02_19260)	4092781..4093431	-	651	WP_233744139.1	type I restriction enzyme HsdR N-terminal domain-containing protein	-
KIK02_RS19120 (KIK02_19265)	4093511..4093765	+	255	WP_233744140.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KIK02_RS19125 (KIK02_19270)	4093762..4094184	+	423	WP_233744141.1	type II toxin-antitoxin system VapC family toxin	Toxin
KIK02_RS19130 (KIK02_19275)	4094203..4097256	-	3054	WP_233744142.1	aminomethyl-transferring glycine dehydrogenase	-
KIK02_RS19135 (KIK02_19280)	4097563..4097832	+	270	WP_233744143.1	hypothetical protein	-
KIK02_RS19140 (KIK02_19285)	4097829..4098512	+	684	WP_233744144.1	ABC transporter ATP-binding protein	-
KIK02_RS19145 (KIK02_19290)	4098631..4099182	+	552	WP_233744145.1	NYN domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4090653..4091183	530

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-131)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-56)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15947.53 Da
• Isoelectric Point: 4.9439

>T203331 WP_233744141.1 NZ_CP075171:4093762-4094184 [Leptodesmis sichuanensis PKUAC-SCTA121]
MKYLLDTCVISELIAKSPNQKVIDWVDQLEEMQIYLSVVTIGEICKGIAKLTDSQRKNELTHWLHQELLVRFADRILPID
VEVMLTWGELTGNLERTGKKIPAVDSLIAAIALQNQLHLVTRNEADFREAGIIVVNPWNQ

Nucleotide

• Length: 423 bp

>T203331 NZ_CP099914:170998-171150 [Escherichia albertii]
ATGCCGCAGAAATATAGATTGCTTTCTTTAATAGTGATTTGTTTCACGCTTTTATTTTTCACCTGGATGATAAGAGATTC
ACTGTGTGAATTGCATATTAAACAGGGGAGTTATGAGCTGGCGGCGTTTTTAGCCTGCAATTTGAAAGAGTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9564.94 Da
• Isoelectric Point: 7.0108

>AT203331 WP_233744140.1 NZ_CP075171:4093511-4093765 [Leptodesmis sichuanensis PKUAC-SCTA121]
MSEVWQLQTAKNKFSEVVEQAIQHGPQVITRRGNEVAVVLSYADYRKLTTPQTKLSVFFRESPLAKVELDLTRDVSAARD
DLML

Nucleotide

• Length: 255 bp

>AT203331 NZ_CP099914:c170947-170893 [Escherichia albertii]
GTTCAGGCATAGGGTAGCCTCACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTG