Detailed information of TA system TA203330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/DUF433(antitoxin)
• Location: 3570014..3570471
• Replicon: chromosome
• Accession: NZ_CP075171
• Organism: Leptodesmis sichuanensis PKUAC-SCTA121

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KIK02_RS16590
• Protein ID: WP_233743698.1
• Coordinates: 3570014..3570241 (-)
• Length: 76 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KIK02_RS16595
• Protein ID: WP_233743699.1
• Coordinates: 3570238..3570471 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIK02_RS16545 (KIK02_16675)	3565441..3566634	+	1194	WP_233743693.1	hypothetical protein	-
KIK02_RS16550 (KIK02_16680)	3566649..3566963	-	315	WP_233743694.1	hypothetical protein	-
KIK02_RS16555 (KIK02_16685)	3566957..3567295	-	339	WP_233743695.1	hypothetical protein	-
KIK02_RS16560 (KIK02_16690)	3567289..3567603	-	315	WP_233743696.1	hypothetical protein	-
KIK02_RS16565 (KIK02_16695)	3567597..3567911	-	315	WP_233743696.1	hypothetical protein	-
KIK02_RS16570 (KIK02_16700)	3568266..3568544	+	279	WP_233743697.1	hypothetical protein	-
KIK02_RS16575 (KIK02_16705)	3568576..3568764	-	189	Protein_3300	hypothetical protein	-
KIK02_RS16580 (KIK02_16710)	3568791..3569330	-	540	WP_233745175.1	transposase	-
KIK02_RS24875 (KIK02_16715)	3569234..3569518	-	285	WP_233745176.1	hypothetical protein	-
KIK02_RS16585 (KIK02_16720)	3569515..3569985	-	471	WP_233743509.1	hypothetical protein	-
KIK02_RS16590 (KIK02_16725)	3570014..3570241	-	228	WP_233743698.1	DUF5615 family PIN-like protein	Toxin
KIK02_RS16595 (KIK02_16730)	3570238..3570471	-	234	WP_233743699.1	DUF433 domain-containing protein	Antitoxin
KIK02_RS16600 (KIK02_16735)	3570629..3571801	-	1173	WP_233743700.1	ISAs1 family transposase	-
KIK02_RS16605 (KIK02_16740)	3572025..3572633	+	609	WP_233743701.1	GUN4 domain-containing protein	-
KIK02_RS16610 (KIK02_16745)	3572725..3572979	+	255	WP_233743702.1	hypothetical protein	-
KIK02_RS16615 (KIK02_16750)	3572976..3573323	+	348	WP_233743703.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KIK02_RS16620 (KIK02_16755)	3573217..3573633	-	417	WP_233749044.1	hypothetical protein	-
KIK02_RS16625 (KIK02_16760)	3573627..3573941	-	315	WP_233743704.1	hypothetical protein	-
KIK02_RS16630 (KIK02_16765)	3573935..3574249	-	315	WP_233743705.1	hypothetical protein	-
KIK02_RS16635 (KIK02_16770)	3574243..3574911	-	669	WP_233743706.1	hypothetical protein	-
KIK02_RS16640 (KIK02_16775)	3575007..3575219	-	213	WP_233743707.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3570629..3571801	1172

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Domain of unknown function DUF5615 (1-52)

Antitoxin

• Protein of unknown function DUF433 (11-66)

Sequences

Toxin

Protein

• Length: 76 a.a.
• Molecular weight: 8352.64 Da
• Isoelectric Point: 5.0001

>T203330 WP_233743698.1 NZ_CP075171:c3570241-3570014 [Leptodesmis sichuanensis PKUAC-SCTA121]
MKIWVDAQLPPTLANWLSESFGLEAYALRDLSLRDAKDIEIFEAARAENAVIRSLQINKIPVILAGAERKYPSPG

Nucleotide

• Length: 228 bp

>T203330 NZ_CP099912:c4406354-4405815 [Escherichia albertii]
ATGTCTCATAATTCAGATATCTATAAACTGATCGGAGCGGCCGCAGGTGTTGAAAATGGTCGCTCTGAAGCATCCTTAAA
CTCTACTGACAGTCAAGAGCAGGCTTTTGAATCCGCATTTGAGCCGTCTAATCATGAACGCGATGATGATTCTTCTTCAG
AAAATAAGGCTATTCTGGAGGAAGAAGAGTTTGGTTCTAATACTGGTGCATTGCATGAGTTTATGCAGCAGAACAGAATG
GACAGTCTTCAAGCTCAGCTTGATATGCTCAAATCACAAGTACGGGATAAGATAGCTGACGCAACTGGTAAAGAAATAGA
CAATGAGCTCCGGACAAAAATGGCATCATTCACGGTTTGGTTTATGTCGTGTTGGTGCTTATTCGTTGTCGCAATGTTTA
CATCGTTTCTTATTGCACATGAAGGAAAGCCTCCAGTTGAAGCGATCGTTGCATTACTAGGTACAAGTACAATTAGTATT
GTTGGTTTGGTTGTTAGTGGATTGTTCAAATCAAGAAAAGATAGTGATAAAGAAAAATAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8520.88 Da
• Isoelectric Point: 4.6320

>AT203330 WP_233743699.1 NZ_CP075171:c3570471-3570238 [Leptodesmis sichuanensis PKUAC-SCTA121]
MNSATGLLTRITQTPGQCGGRPCIRGMRIRVTDILEMLAENVSVNEILEDFPDLELADIQACLLFAARRTDFPRLTA

Nucleotide

• Length: 234 bp

>AT203330 NZ_CP099912:c4406850-4406377 [Escherichia albertii]
ATGTACAGTCCGGTACAAATAGCCAATAAATTCATAACGTTGGGAAACCAACATCATAATCCTCTGACCCACATGCAACT
GCAGAAGCTTACTTATATTGCCCATGGTTACTATTTAGCATTAACAGGTAAGCCTTTGCTCAATGAATGTGTCTCCGCGT
GGAAGTACGGTCCAGTTATTCCTGGAATGTACGATGCTTTCAAAGACTATGGGAATAAACCTGTTACGAATGTAGCAGTA
GCTCCTTTTGGTGGCATCGTTACTATGGATCCGCAAGCAGAGAGCATTATCGGGGCTGTTTATAAGTTTTACGGCTCGAA
AAATGGAATTGAGTTATCAACTCTAACTCATATGCCAGGTACGCCTTGGTCACAAGCTTATAATGGTATTGGCTCGTCGA
TCATCTCAAATGATGCGATCAAGGCTTATTATCATGATTTATTGAATAACCGACAGCAATGTCAGGGCCTCTGA