Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3845873..3846527 Replicon chromosome
Accession NC_002655
Organism Escherichia coli O157:H7 str. EDL933

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag Z_RS19855 Protein ID WP_000244781.1
Coordinates 3845873..3846280 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag Z_RS19860 Protein ID WP_000354046.1
Coordinates 3846261..3846527 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Z_RS19835 (Z4230) 3841830..3843563 - 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -
Z_RS19840 (Z4231) 3843569..3844279 - 711 WP_000715215.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
Z_RS19845 (Z4232) 3844304..3845200 - 897 WP_000806640.1 site-specific tyrosine recombinase XerD -
Z_RS19850 (Z4233) 3845312..3845833 + 522 WP_001055874.1 flavodoxin FldB -
Z_RS19855 (Z4234) 3845873..3846280 - 408 WP_000244781.1 protein YgfX Toxin
Z_RS19860 (Z4235) 3846261..3846527 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
Z_RS19865 (Z4236) 3846770..3847750 + 981 WP_000886053.1 tRNA-modifying protein YgfZ -
Z_RS19870 (Z4237) 3847827..3848486 - 660 WP_000250274.1 hemolysin III family protein -
Z_RS19875 (Z4238) 3848650..3848961 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
Z_RS19880 (Z4239) 3849006..3850439 + 1434 WP_024201377.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T20331 WP_000244781.1 NC_002655:c3846280-3845873 [Escherichia coli O157:H7 str. EDL933]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T20331 NC_002655:c3846280-3845873 [Escherichia coli O157:H7 str. EDL933]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT20331 WP_000354046.1 NC_002655:c3846527-3846261 [Escherichia coli O157:H7 str. EDL933]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT20331 NC_002655:c3846527-3846261 [Escherichia coli O157:H7 str. EDL933]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References