Detailed information of TA system TA203305

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2494791..2495915
• Replicon: chromosome
• Accession: NZ_CP075169
• Organism: Sodalis ligni strain dw23

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: GTU79_RS11625
• Protein ID: WP_214513952.1
• Coordinates: 2495340..2495915 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: GTU79_RS11620
• Protein ID: WP_203521806.1
• Coordinates: 2494791..2495336 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTU79_RS11595 (GTU79_11590)	2490054..2490458	-	405	WP_203521809.1	VOC family protein	-
GTU79_RS11600 (GTU79_11595)	2490634..2492091	-	1458	WP_253073551.1	AMP-binding protein	-
GTU79_RS11605 (GTU79_11600)	2492060..2492956	-	897	WP_253073552.1	AMP-binding protein	-
GTU79_RS11610 (GTU79_11605)	2492984..2493773	-	790	Protein_2286	IS21-like element helper ATPase IstB	-
GTU79_RS11615 (GTU79_11610)	2493770..2494792	-	1023	WP_203520365.1	IS21 family transposase	-
GTU79_RS11620 (GTU79_11615)	2494791..2495336	+	546	WP_203521806.1	helix-turn-helix domain-containing protein	Antitoxin
GTU79_RS11625 (GTU79_11620)	2495340..2495915	+	576	WP_214513952.1	PIN domain-containing protein	Toxin
GTU79_RS11630 (GTU79_11625)	2496432..2498048	-	1617	WP_203521804.1	IS66 family transposase	-
GTU79_RS11635 (GTU79_11630)	2498115..2498450	-	336	WP_214513953.1	IS66 family insertion sequence element accessory protein TnpB	-
GTU79_RS30250	2498444..2498860	-	417	WP_253073553.1	hypothetical protein	-
GTU79_RS30255	2499084..2499272	-	189	Protein_2293	ISL3 family transposase	-
GTU79_RS30260	2499800..2500066	-	267	WP_253073554.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2494791..2514578	19787
	-	inside	IScluster/Tn	-	-	2492984..2502806	9822

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-115)

Antitoxin

• Helix-turn-helix domain, group 17 (111-159)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21358.83 Da
• Isoelectric Point: 4.9685

>T203305 WP_214513952.1 NZ_CP075169:2495340-2495915 [Sodalis ligni]
MMHSPYPVVLDACVLYPARLRDLLMHLGLAGLYQPKWSAVIHDEWRCNLLLQRSDINPEALARTTDLMNLALPDANVTGF
ETIIEGISLPDPDDRHVLAAAIRSNADVIVTLNLKDFPSGVLAGFGIEALHLDDFISDLFDLNHSVALNAVRLQRNALKN
PPMTTEEFLEMLLKQGLPMTVKALRNYQFMI

Nucleotide

• Length: 576 bp

>T203305 NZ_CP099910:2145927-2146029 [Escherichia albertii]
GCAAGGCAACTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAAAACA
GGAATCGTTTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19854.94 Da
• Isoelectric Point: 6.6996

>AT203305 WP_203521806.1 NZ_CP075169:2494791-2495336 [Sodalis ligni]
MGSFLKLPLLPQVGQIYIGRWVIITSVATNTTLTGLALPAKHEIETAIRGQRDLAAFLSTKLETQQILIKDADDVSHQIE
LPTAALTLLMNVLGELAEGNAVQVVPVHAELTTQEAANILNVSRPHMVKLLEDGHLPHHKTGRHRRVLFADLMAYKNERD
ATSQEAMQKLAAQAQELGLGY

Nucleotide

• Length: 546 bp

>AT203305 NZ_CP099910:c2146031-2145799 [Escherichia albertii]
ATAAAAAGAGACCGAAAACGATTCCTGTTTTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTCAGTTGCCTTGCTCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCGTACAAAATGGTCAG
TAAAAAGCGCGTCGGCCATCGTCTTAAATGTTAAAAACCGCCCGTTCTGGTGAAAGAACTGTGGCGGTTTTTT