Detailed information of TA system TA203216

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1925890..1926480
• Replicon: chromosome
• Accession: NZ_CP075137
• Organism: Salmonella enterica strain CFSAN044885

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A8E7N510
• Locus tag: A7S07_RS09135
• Protein ID: WP_070793950.1
• Coordinates: 1926148..1926480 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A5X3P062
• Locus tag: A7S07_RS09130
• Protein ID: WP_023216880.1
• Coordinates: 1925890..1926147 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7S07_RS09115 (1922551)	1922551..1924506	-	1956	WP_070793949.1	hypothetical protein	-
A7S07_RS09120 (1924866)	1924866..1925339	+	474	WP_023216882.1	hypothetical protein	-
A7S07_RS09125 (1925336)	1925336..1925542	+	207	WP_024147225.1	helix-turn-helix transcriptional regulator	-
A7S07_RS09130 (1925890)	1925890..1926147	+	258	WP_023216880.1	antitoxin PemI	Antitoxin
A7S07_RS09135 (1926148)	1926148..1926480	+	333	WP_070793950.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
A7S07_RS09140 (1927327)	1927327..1928211	+	885	WP_023216878.1	integrase domain-containing protein	-
A7S07_RS09150 (1929241)	1929241..1930503	-	1263	WP_023216875.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11765.58 Da
• Isoelectric Point: 10.2965

>T203216 WP_070793950.1 NZ_CP075137:1926148-1926480 [Salmonella enterica]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSQASFNKLTRLPVIVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARNGKRLERIPDAVVNEVLARLEAILS

Nucleotide

• Length: 333 bp

>T203216 NZ_CP099895:c811316-810939 [Escherichia albertii]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTCGCGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCGGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACCGAACAGTAAATAACATTACTCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9356.83 Da
• Isoelectric Point: 4.4488

>AT203216 WP_023216880.1 NZ_CP075137:1925890-1926147 [Salmonella enterica]
MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT203216 NZ_CP099895:c811774-811406 [Escherichia albertii]
GTGTCAGACACACTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTTGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A5X3P062