Detailed information of TA system TA203210

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 10577..11130
• Replicon: plasmid pCFSAN044888
• Accession: NZ_CP075136
• Organism: Salmonella enterica strain CFSAN044888

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A3R0G118
• Locus tag: A7S10_RS23660
• Protein ID: WP_058645035.1
• Coordinates: 10577..10891 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A6P1V7Y5
• Locus tag: A7S10_RS23665
• Protein ID: WP_025861853.1
• Coordinates: 10894..11130 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7S10_RS23625 (A7S10_023615)	5916..6128	-	213	Protein_8	sprT domain-containing protein	-
A7S10_RS23630 (A7S10_023620)	6208..6648	-	441	Protein_9	AAA family ATPase	-
A7S10_RS23635 (A7S10_023625)	6763..6909	-	147	WP_057394955.1	colicin release lysis protein	-
A7S10_RS23640 (A7S10_023630)	6993..7250	-	258	WP_070794402.1	bacteriocin immunity protein	-
A7S10_RS23645 (A7S10_023635)	7253..9004	-	1752	WP_058645033.1	colicin-like bacteriocin tRNase domain-containing protein	-
A7S10_RS23650 (A7S10_023640)	9469..9824	-	356	Protein_13	SprT-like domain-containing protein	-
A7S10_RS24025	10243..10356	-	114	Protein_14	XRE family transcriptional regulator	-
A7S10_RS23655 (A7S10_023645)	10372..10536	-	165	WP_165801018.1	hypothetical protein	-
A7S10_RS23660 (A7S10_023650)	10577..10891	-	315	WP_058645035.1	CcdB family protein	Toxin
A7S10_RS23665 (A7S10_023655)	10894..11130	-	237	WP_025861853.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
A7S10_RS23670 (A7S10_023660)	11239..11670	-	432	WP_058645036.1	hypothetical protein	-
A7S10_RS23675 (A7S10_023665)	12477..13184	-	708	WP_254891021.1	RepA protein	-
A7S10_RS23680 (A7S10_023670)	13772..14131	-	360	WP_057517074.1	helix-turn-helix transcriptional regulator	-
A7S10_RS23685 (A7S10_023675)	14381..14836	+	456	WP_058645037.1	transcription termination/antitermination NusG family protein	-
A7S10_RS23690 (A7S10_023680)	14908..15123	+	216	WP_004178170.1	hypothetical protein	-
A7S10_RS23695 (A7S10_023685)	15128..15346	+	219	WP_004178172.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..26158	26158

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11542.39 Da
• Isoelectric Point: 5.8036

>T203210 WP_058645035.1 NZ_CP075136:c10891-10577 [Salmonella enterica]
MQFTVYSNTGKSTVYPLLLDVTSDIIGQLNRRIVIPLLPVEKYPGSTRPERLVPLVRLTDDKEYAVMTHEMASIPARSLG
AAFCDASPYRTEVKAAIDFLLDGI

Nucleotide

• Length: 315 bp

>T203210 NZ_CP099894:77501-77806 [Escherichia albertii]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGGGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8907.84 Da
• Isoelectric Point: 4.8040

>AT203210 WP_025861853.1 NZ_CP075136:c11130-10894 [Salmonella enterica]
MATTQRATQSVTMTVERALLSRAREAGINLSATLTAALDAELRQYEAKKWQEENNEALEALNRFHDEHGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT203210 NZ_CP099894:77281-77499 [Escherichia albertii]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGCGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A3R0G118

Antitoxin

• AlphaFold DB: A0A6P1V7Y5