Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2062786..2063424 Replicon chromosome
Accession NC_002655
Organism Escherichia coli O157:H7 str. EDL933

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag Z_RS10715 Protein ID WP_000813794.1
Coordinates 2063248..2063424 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag Z_RS10710 Protein ID WP_085953820.1
Coordinates 2062786..2063202 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Z_RS10690 (Z2277) 2057938..2058879 - 942 WP_001251320.1 ABC transporter permease -
Z_RS10695 (Z2278) 2058880..2059893 - 1014 WP_000220402.1 ABC transporter ATP-binding protein -
Z_RS10700 (Z2279) 2059911..2061056 - 1146 WP_000047432.1 ABC transporter substrate-binding protein -
Z_RS10705 (Z2280) 2061301..2062707 - 1407 WP_000760656.1 PLP-dependent aminotransferase family protein -
Z_RS10710 (Z2281) 2062786..2063202 - 417 WP_085953820.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
Z_RS10715 2063248..2063424 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
Z_RS10720 (Z2283) 2063646..2063876 + 231 WP_000494244.1 YncJ family protein -
Z_RS10725 (Z2284) 2063968..2065929 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
Z_RS10730 (Z2285) 2066002..2066538 - 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
Z_RS10735 (Z2286) 2066591..2067802 + 1212 WP_071525382.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(88-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T20320 WP_000813794.1 NC_002655:c2063424-2063248 [Escherichia coli O157:H7 str. EDL933]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T20320 NC_002655:c2063424-2063248 [Escherichia coli O157:H7 str. EDL933]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15232.60 Da        Isoelectric Point: 4.7386

>AT20320 WP_085953820.1 NC_002655:c2063202-2062786 [Escherichia coli O157:H7 str. EDL933]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAGKALGKELSLVMV

Download         Length: 417 bp

>AT20320 NC_002655:c2063202-2062786 [Escherichia coli O157:H7 str. EDL933]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCAGTCCAGCTCGCGGGAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References