Detailed information of TA system TA020319

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1256680..1257158
• Replicon: chromosome
• Accession: NC_002655
• Organism: Escherichia coli O157:H7 str. EDL933

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: Z_RS06115
• Protein ID: WP_001303876.1
• Coordinates: 1256680..1256967 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: Z_RS06120
• Protein ID: WP_000536233.1
• Coordinates: 1256967..1257158 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Z_RS06080 (Z1324)	1251828..1254299	-	2472	WP_000034475.1	exonuclease	-
Z_RS06085 (Z1325)	1254393..1254584	-	192	WP_001098307.1	DUF1482 family protein	-
Z_RS06090 (Z1326)	1254581..1254769	-	189	WP_000413705.1	cell division inhibition protein DicB	-
Z_RS06100 (Z1328)	1255344..1255529	+	186	WP_001133046.1	hypothetical protein	-
Z_RS06105 (Z1329)	1255716..1256105	-	390	WP_000394511.1	hypothetical protein	-
Z_RS06110 (Z1331)	1256247..1256402	-	156	WP_000379575.1	DUF1391 family protein	-
Z_RS06115	1256680..1256967	-	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Z_RS06120 (Z1332)	1256967..1257158	-	192	WP_000536233.1	hypothetical protein	Antitoxin
Z_RS06125 (Z1333)	1257186..1257587	-	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
Z_RS06130 (Z1334)	1257696..1257968	+	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
Z_RS06135 (Z1335)	1257952..1258377	+	426	WP_000693855.1	Rha family transcriptional regulator	-
Z_RS29390	1258584..1259039	-	456	WP_000273724.1	hypothetical protein	-
Z_RS06145 (Z1337)	1259118..1260209	+	1092	WP_001205823.1	hypothetical protein	-
Z_RS06150 (Z1338)	1260216..1260962	+	747	WP_000788751.1	ATP-binding protein	-
Z_RS06155 (Z1339)	1260984..1261754	+	771	WP_000450992.1	DUF1627 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	espFu/tccP	1250319..1295622	45303

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T20319 WP_001303876.1 NC_002655:c1256967-1256680 [Escherichia coli O157:H7 str. EDL933]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T20319 NC_002655:c1256967-1256680 [Escherichia coli O157:H7 str. EDL933]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT20319 WP_000536233.1 NC_002655:c1257158-1256967 [Escherichia coli O157:H7 str. EDL933]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT20319 NC_002655:c1257158-1256967 [Escherichia coli O157:H7 str. EDL933]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA