Detailed information of TA system TA020318

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1002725..1003430
• Replicon: chromosome
• Accession: NC_002655
• Organism: Escherichia coli O157:H7 str. EDL933

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q8X3N0
• Locus tag: Z_RS04775
• Protein ID: WP_000539519.1
• Coordinates: 1003044..1003430 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: Z_RS04770
• Protein ID: WP_001280945.1
• Coordinates: 1002725..1003054 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Z_RS04755 (Z1056)	997882..998793	+	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-
Z_RS04760 (Z1057)	998971..1001319	+	2349	WP_000950320.1	EAL domain-containing protein	-
Z_RS04765 (Z1058)	1001327..1002655	+	1329	WP_000086905.1	GGDEF domain-containing protein	-
Z_RS04770 (Z1059)	1002725..1003054	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
Z_RS04775 (Z1060)	1003044..1003430	-	387	WP_000539519.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Z_RS04780 (Z1061)	1003656..1004981	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
Z_RS04785 (Z1062)	1005194..1005577	+	384	WP_000497137.1	biofilm formation regulator BssR	-
Z_RS04790 (Z1063)	1005688..1006803	+	1116	WP_000554959.1	aldose sugar dehydrogenase YliI	-
Z_RS04795 (Z1064)	1006800..1007426	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14279.42 Da
• Isoelectric Point: 9.9296

>T20318 WP_000539519.1 NC_002655:c1003430-1003044 [Escherichia coli O157:H7 str. EDL933]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGSKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T20318 NC_002655:c1003430-1003044 [Escherichia coli O157:H7 str. EDL933]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAAGCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT20318 WP_001280945.1 NC_002655:c1003054-1002725 [Escherichia coli O157:H7 str. EDL933]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT20318 NC_002655:c1003054-1002725 [Escherichia coli O157:H7 str. EDL933]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA