Detailed information of TA system TA203173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 321380..321966
• Replicon: chromosome
• Accession: NZ_CP075134
• Organism: Salmonella enterica subsp. enterica serovar Saintpaul strain CFSAN044909

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q57IR9
• Locus tag: A7S31_RS01475
• Protein ID: WP_000174963.1
• Coordinates: 321598..321966 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: V7IU48
• Locus tag: A7S31_RS01470
• Protein ID: WP_001522145.1
• Coordinates: 321380..321601 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7S31_RS01445 (316401)	316401..317510	+	1110	WP_000822976.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
A7S31_RS01450 (317570)	317570..318496	+	927	WP_001522035.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
A7S31_RS01455 (318493)	318493..319770	+	1278	WP_000803764.1	branched chain amino acid ABC transporter permease LivM	-
A7S31_RS01460 (319767)	319767..320534	+	768	WP_000082080.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
A7S31_RS01465 (320536)	320536..321249	+	714	WP_000416114.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
A7S31_RS01470 (321380)	321380..321601	+	222	WP_001522145.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
A7S31_RS01475 (321598)	321598..321966	+	369	WP_000174963.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
A7S31_RS01480 (322225)	322225..323541	+	1317	WP_000624747.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
A7S31_RS01485 (323646)	323646..324533	+	888	WP_000099303.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
A7S31_RS01490 (324530)	324530..325375	+	846	WP_000572196.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
A7S31_RS01495 (325378)	325378..326448	+	1071	WP_000907839.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	316401..327185	10784

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-49)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13573.85 Da
• Isoelectric Point: 6.7252

>T203173 WP_000174963.1 NZ_CP075134:321598-321966 [Salmonella enterica subsp. enterica serovar Saintpaul]
MTLQLISAEEIIQFHDRLLRVTPGVTGMPDPGRAEALMYRVLNQIEYEGVTDVWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGISLAANPDFVDMTVDAAAGRLTLEQIAVRLRA

Nucleotide

• Length: 369 bp

>T203173 NZ_CP099887:3992578-3992919 [Escherichia albertii]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCACCCAACCACCCGCCGCCGAAGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAAGCAGGTGCAGGAGTTTATTGGGGTAATTAC
AGGTAAACAGAAAGTCGCCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8166.17 Da
• Isoelectric Point: 5.0775

>AT203173 WP_001522145.1 NZ_CP075134:321380-321601 [Salmonella enterica subsp. enterica serovar Saintpaul]
MRTVNYSEARQNLAEVLESAVTAGPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGNELRELADK

Nucleotide

• Length: 222 bp

>AT203173 NZ_CP099887:c3992583-3992507 [Escherichia albertii]
AGTCATGATTACTATTCTCCATAAATAGTGATTGTGATGAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A5H6KCD5