Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 546158..546776 Replicon chromosome
Accession NC_002655
Organism Escherichia coli O157:H7 str. EDL933

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag Z_RS02630 Protein ID WP_001291435.1
Coordinates 546158..546376 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag Z_RS02635 Protein ID WP_000344800.1
Coordinates 546402..546776 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Z_RS02600 (Z0565) 541445..542017 + 573 WP_000779831.1 YbaY family lipoprotein -
Z_RS02605 (Z0566) 542050..542361 - 312 WP_000409911.1 MGMT family protein -
Z_RS02610 (Z0568) 542740..543093 + 354 WP_000878140.1 DUF1428 domain-containing protein -
Z_RS02615 (Z0569) 543135..544685 - 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
Z_RS02620 (Z0570) 544849..545319 - 471 WP_000136192.1 YlaC family protein -
Z_RS02625 (Z0571) 545435..545986 - 552 WP_000102553.1 maltose O-acetyltransferase -
Z_RS02630 (Z0573) 546158..546376 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
Z_RS02635 (Z0574) 546402..546776 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
Z_RS02640 (Z0576) 547322..550471 - 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
Z_RS02645 (Z0578) 550494..551687 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T20317 WP_001291435.1 NC_002655:c546376-546158 [Escherichia coli O157:H7 str. EDL933]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T20317 NC_002655:c546376-546158 [Escherichia coli O157:H7 str. EDL933]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT20317 WP_000344800.1 NC_002655:c546776-546402 [Escherichia coli O157:H7 str. EDL933]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT20317 NC_002655:c546776-546402 [Escherichia coli O157:H7 str. EDL933]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References