Detailed information of TA system TA203152

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 21773..22326
• Replicon: plasmid pCFSAN044921_2
• Accession: NZ_CP075131
• Organism: Salmonella enterica subsp. enterica serovar Rubislaw strain CFSAN044921

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A3R0G118
• Locus tag: A7S43_RS24415
• Protein ID: WP_058645035.1
• Coordinates: 22012..22326 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A6P1V7Y5
• Locus tag: A7S43_RS24410
• Protein ID: WP_025861853.1
• Coordinates: 21773..22009 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7S43_RS24375 (A7S43_24365)	16856..17566	-	711	WP_058645038.1	lytic transglycosylase domain-containing protein	-
A7S43_RS24755	17565..17687	+	123	WP_254891022.1	hypothetical protein	-
A7S43_RS24380 (A7S43_24370)	17684..17902	-	219	WP_004178172.1	hypothetical protein	-
A7S43_RS24385 (A7S43_24375)	17907..18122	-	216	WP_004178170.1	hypothetical protein	-
A7S43_RS24390 (A7S43_24380)	18194..18649	-	456	WP_058645037.1	transcription termination/antitermination NusG family protein	-
A7S43_RS24395 (A7S43_24385)	18899..19258	+	360	WP_057517074.1	helix-turn-helix transcriptional regulator	-
A7S43_RS24400 (A7S43_24390)	19719..20426	+	708	WP_254891021.1	RepA protein	-
A7S43_RS24405 (A7S43_24395)	21233..21664	+	432	WP_058645036.1	hypothetical protein	-
A7S43_RS24410 (A7S43_24400)	21773..22009	+	237	WP_025861853.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
A7S43_RS24415 (A7S43_24405)	22012..22326	+	315	WP_058645035.1	CcdB family protein	Toxin
A7S43_RS24420 (A7S43_24410)	22367..22531	+	165	WP_165801018.1	hypothetical protein	-
A7S43_RS24760	22547..22660	+	114	Protein_34	XRE family transcriptional regulator	-
A7S43_RS24425 (A7S43_24415)	23079..23434	+	356	Protein_35	SprT-like domain-containing protein	-
A7S43_RS24430 (A7S43_24420)	23899..25650	+	1752	WP_058645033.1	colicin-like bacteriocin tRNase domain-containing protein	-
A7S43_RS24435 (A7S43_24425)	25653..25910	+	258	WP_070794402.1	bacteriocin immunity protein	-
A7S43_RS24440 (A7S43_24430)	25994..26140	+	147	WP_057394955.1	colicin release lysis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..26158	26158

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11542.39 Da
• Isoelectric Point: 5.8036

>T203152 WP_058645035.1 NZ_CP075131:22012-22326 [Salmonella enterica subsp. enterica serovar Rubislaw]
MQFTVYSNTGKSTVYPLLLDVTSDIIGQLNRRIVIPLLPVEKYPGSTRPERLVPLVRLTDDKEYAVMTHEMASIPARSLG
AAFCDASPYRTEVKAAIDFLLDGI

Nucleotide

• Length: 315 bp

>T203152 NZ_CP099886:3684544-3684646 [Escherichia albertii]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAAAACA
GGAATCGTTTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8907.84 Da
• Isoelectric Point: 4.8040

>AT203152 WP_025861853.1 NZ_CP075131:21773-22009 [Salmonella enterica subsp. enterica serovar Rubislaw]
MATTQRATQSVTMTVERALLSRAREAGINLSATLTAALDAELRQYEAKKWQEENNEALEALNRFHDEHGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT203152 NZ_CP099886:c3684648-3684416 [Escherichia albertii]
ATAAAAAGAGACCGAAAACGATTCCTGTTTTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCGTACAAAATGGTCAG
TAAAAAGCGCGTCGGCCATCGTCTTAAATGTTAAAAACCGCCCGTTCTGGTGAAAGAACTGTGGCGGTTTTTT

Structures

Toxin

• AlphaFold DB: A0A3R0G118

Antitoxin

• AlphaFold DB: A0A6P1V7Y5