Detailed information of TA system TA020312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 549122..549754
• Replicon: chromosome
• Accession: NC_002570
• Organism: Bacillus halodurans C-125

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q9KFF7
• Locus tag: AYT26_RS02800
• Protein ID: WP_010896700.1
• Coordinates: 549404..549754 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: AYT26_RS02795
• Protein ID: WP_173355321.1
• Coordinates: 549122..549400 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYT26_RS02775	545016..545762	-	747	WP_010896695.1	rhomboid family intramembrane serine protease	-
AYT26_RS02780	545984..546343	+	360	WP_010896696.1	holo-ACP synthase	-
AYT26_RS02785	546530..547534	+	1005	WP_010896697.1	outer membrane lipoprotein carrier protein LolA	-
AYT26_RS02790	547762..548928	+	1167	WP_010896698.1	alanine racemase	-
AYT26_RS02795	549122..549400	+	279	WP_173355321.1	antitoxin	Antitoxin
AYT26_RS02800	549404..549754	+	351	WP_010896700.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AYT26_RS02805	549935..550750	+	816	WP_010896701.1	RsbT co-antagonist protein RsbRA	-
AYT26_RS02810	550765..551121	+	357	WP_010896702.1	STAS domain-containing protein	-
AYT26_RS02815	551126..551545	+	420	WP_010896703.1	anti-sigma regulatory factor	-
AYT26_RS02820	551560..552573	+	1014	WP_010896704.1	PP2C family protein-serine/threonine phosphatase	-
AYT26_RS02825	552645..552977	+	333	WP_010896705.1	STAS domain-containing protein	-
AYT26_RS02830	552974..553462	+	489	WP_010896706.1	anti-sigma B factor RsbW	-
AYT26_RS02835	553428..554207	+	780	WP_041820201.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12904.97 Da
• Isoelectric Point: 6.4662

>T20312 WP_010896700.1 NC_002570:549404-549754 [Bacillus halodurans C-125]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKRYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDDMMSKVNDALLISLGLIDF

Nucleotide

• Length: 351 bp

>T20312 NC_002570:549404-549754 [Bacillus halodurans C-125]
TTGATTGTAAAGCGAGGCGACGTGTACTTTGCCGACTTGTCTCCTGTCGTTGGTTCTGAACAAGGCGGAGTTCGGCCGGT
TCTCGTCATACAAAATGATATTGGAAATCGGTTCAGTCCCACTGTCATTGTGGCTGCAATCACCGCACAAATCCAAAAGG
CGAAGTTGCCGACTCATGTAGAAATTAATGCGAAGCGCTATGGTTTCGATCGCGATTCCGTTATTTTGCTTGAGCAAATT
CGAACAATTGATAAACAGAGGCTGACGGACAAGATTACCCATCTTGACGATGATATGATGTCGAAGGTAAATGATGCCCT
GCTTATTAGTTTAGGTCTAATCGACTTCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10676.23 Da
• Isoelectric Point: 8.5491

>AT20312 WP_173355321.1 NC_002570:549122-549400 [Bacillus halodurans C-125]
VSESSTKRIVVNLPQHLLNEVDGVIKQEKVNRSEFFSQATKMYLRERKKRQIREKMQQGYLEMAKINLNIASEAFLAEEE
AEHTLDRLVSGV

Nucleotide

• Length: 279 bp

>AT20312 NC_002570:549122-549400 [Bacillus halodurans C-125]
GTGTCAGAATCAAGCACAAAACGAATTGTCGTGAATTTACCACAACATTTGCTAAATGAAGTGGACGGCGTTATTAAACA
AGAGAAGGTTAATCGGAGTGAGTTTTTCTCTCAAGCCACAAAAATGTATTTACGTGAACGAAAAAAACGTCAAATTCGTG
AAAAAATGCAGCAAGGCTACTTAGAGATGGCGAAAATTAATCTTAATATTGCGTCTGAAGCTTTTTTGGCTGAGGAGGAA
GCTGAGCACACCCTAGATCGCTTAGTAAGTGGGGTGTAG

Structures

Toxin

• AlphaFold DB: Q9KFF7