Detailed information of TA system TA203076

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 14497..15022
• Replicon: plasmid pCFSAN051827_1
• Accession: NZ_CP075123
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain CFSAN051827

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: I3W3D5
• Locus tag: BCA48_RS22695
• Protein ID: WP_001159863.1
• Coordinates: 14497..14802 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7S5D0
• Locus tag: BCA48_RS22700
• Protein ID: WP_000813641.1
• Coordinates: 14804..15022 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCA48_RS22655 (BCA48_22660)	9876..10436	-	561	WP_000900095.1	inverse autotransporter beta domain-containing protein	-
BCA48_RS22660 (BCA48_22665)	10592..10879	+	288	WP_071530243.1	hypothetical protein	-
BCA48_RS22665 (BCA48_22670)	11156..11641	-	486	WP_000905606.1	membrane protein	-
BCA48_RS22670 (BCA48_22675)	11635..12123	-	489	WP_001075507.1	CaiF/GrlA family transcriptional regulator	-
BCA48_RS22675 (BCA48_22680)	12148..12861	-	714	WP_000545756.1	EAL domain-containing protein	-
BCA48_RS22680 (BCA48_22685)	12870..13652	-	783	WP_000082169.1	site-specific integrase	-
BCA48_RS22685 (BCA48_22690)	13687..14208	-	522	WP_000198608.1	hypothetical protein	-
BCA48_RS22690 (BCA48_22695)	14205..14495	-	291	WP_001266176.1	hypothetical protein	-
BCA48_RS22695 (BCA48_22700)	14497..14802	-	306	WP_001159863.1	type II toxin-antitoxin system toxin CcdB	Toxin
BCA48_RS22700 (BCA48_22705)	14804..15022	-	219	WP_000813641.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
BCA48_RS22705 (BCA48_22710)	15698..16219	+	522	WP_077681952.1	hypothetical protein	-
BCA48_RS22710 (BCA48_22715)	16263..16691	-	429	Protein_23	hypothetical protein	-
BCA48_RS22715 (BCA48_22720)	16703..16998	+	296	Protein_24	cytoplasmic protein	-
BCA48_RS22720 (BCA48_22725)	17492..18481	+	990	WP_000461382.1	RepB family plasmid replication initiator protein	-
BCA48_RS22725 (BCA48_22730)	18850..18969	-	120	Protein_26	recombinase	-
BCA48_RS22730 (BCA48_22735)	19022..19408	-	387	WP_000751876.1	YgiW/YdeI family stress tolerance OB fold protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	spvB / spvC / fdeC / pefB / pefA / pefC / pefD / rck / rck	1..61453	61453

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11587.48 Da
• Isoelectric Point: 6.9787

>T203076 WP_001159863.1 NZ_CP075123:c14802-14497 [Salmonella enterica subsp. enterica serovar Enteritidis]
MQFKVYTCKRESRYRLFVDVQSDIIDTPGRRMAVPLVSARLLSEKVPRDLYPVMHIGDEPYRLLTTDMTSVPATVIGEEV
ADLSLRENDIKNAINLMFRGI

Nucleotide

• Length: 306 bp

>T203076 NZ_CP099874:2117217-2117320 [Escherichia albertii]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAAAAC
AGGAATCGTTTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8314.16 Da
• Isoelectric Point: 4.5203

>AT203076 WP_000813641.1 NZ_CP075123:c15022-14804 [Salmonella enterica subsp. enterica serovar Enteritidis]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADDNRNW

Nucleotide

• Length: 219 bp

>AT203076 NZ_CP099874:c2117322-2117090 [Escherichia albertii]
ATAAAAAGAGACCGAAAACGATTCCTGTTTTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCGTACAAAATGGTCAG
TAAAAAGCGCGTCGGCCATCGTCTTAAATGTTAAAAACCGCCCGTTCTGGTGAAAGAACTGTGGCGGTTTTTT

Structures

Toxin

• AlphaFold DB: I3W3D5

Antitoxin

• AlphaFold DB: A0A656ICA6