Detailed information of TA system TA203050

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 200177..200937
• Replicon: chromosome
• Accession: NZ_CP075122
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain CFSAN051827

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A1R2W0C7
• Locus tag: BCA48_RS00930
• Protein ID: WP_000533912.1
• Coordinates: 200452..200937 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: M7RHS4
• Locus tag: BCA48_RS00925
• Protein ID: WP_000965886.1
• Coordinates: 200177..200464 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCA48_RS00905 (195582)	195582..196493	+	912	WP_001168551.1	glycine--tRNA ligase subunit alpha	-
BCA48_RS00910 (196503)	196503..198572	+	2070	WP_001291741.1	glycine--tRNA ligase subunit beta	-
BCA48_RS23245 (198962)	198962..199360	+	399	Protein_181	IS3 family transposase	-
BCA48_RS00920 (199532)	199532..199999	+	468	WP_000702452.1	GNAT family N-acetyltransferase	-
BCA48_RS00925 (200177)	200177..200464	+	288	WP_000965886.1	DUF1778 domain-containing protein	Antitoxin
BCA48_RS00930 (200452)	200452..200937	+	486	WP_000533912.1	GNAT family N-acetyltransferase	Toxin
BCA48_RS00935 (201308)	201308..201847	-	540	WP_000047147.1	copper-binding periplasmic metallochaperone CueP	-
BCA48_RS00940 (202021)	202021..202233	-	213	WP_000014594.1	RNA chaperone/antiterminator CspA	-
BCA48_RS00945 (202521)	202521..202811	-	291	WP_000455790.1	HTH-type transcriptional regulator	-
BCA48_RS00950 (203250)	203250..203960	+	711	WP_000190524.1	DUF3053 domain-containing protein	-
BCA48_RS00955 (204010)	204010..204984	-	975	WP_000804678.1	glyoxylate/hydroxypyruvate reductase GhrB	-
BCA48_RS00960 (205203)	205203..205865	-	663	WP_000747548.1	OmpA family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (31-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-94)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17731.46 Da
• Isoelectric Point: 9.8719

>T203050 WP_000533912.1 NZ_CP075122:200452-200937 [Salmonella enterica subsp. enterica serovar Enteritidis]
VGRVTAPEPLSAFHQVAEFVSGEAVLDDWLKQKGLKNQALGAARTFVVCKKDTKQVAGFYSLATGSVNHTEVTGNLRRNM
PDPIPVIILARLAVDLSFHGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKSSQTQQRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T203050 NZ_CP099868:c4134852-4134511 [Escherichia albertii]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCGCCCGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGACTGAACCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTTGATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTGCGGGCGAGGCAGGCTACAGGTCAGCTGCGTCGCTG
CCATAACACCACGGCACGGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10950.60 Da
• Isoelectric Point: 4.6811

>AT203050 WP_000965886.1 NZ_CP075122:200177-200464 [Salmonella enterica subsp. enterica serovar Enteritidis]
MLYKGCLMKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILETACQAAEKVILDRRVFNFNDEQYEEFINLLDAPVADD
PVIEKLLARKPQWDV

Nucleotide

• Length: 288 bp

>AT203050 NZ_CP099868:c4135218-4134883 [Escherichia albertii]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTTACACCACGCTTCACTGCACGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGCTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCCCCTGCCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Toxin

• AlphaFold DB: A0A1R2W0C7

Antitoxin

• AlphaFold DB: A0A3V2JDX2