Detailed information of TA system TA203043

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 3980531..3981107
• Replicon: chromosome
• Accession: NZ_CP075120
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain CFSAN051882

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: M7RG88
• Locus tag: BCB01_RS19305
• Protein ID: WP_001131963.1
• Coordinates: 3980820..3981107 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: B5R9R5
• Locus tag: BCB01_RS19300
• Protein ID: WP_000063142.1
• Coordinates: 3980531..3980833 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCB01_RS19285 (3977041)	3977041..3979191	+	2151	WP_000379928.1	pyruvate/proton symporter BtsT	-
BCB01_RS19290 (3979286)	3979286..3979489	+	204	WP_000460616.1	YbdD/YjiX family protein	-
BCB01_RS19295 (3979500)	3979500..3980456	+	957	WP_000187843.1	GTPase	-
BCB01_RS19300 (3980531)	3980531..3980833	-	303	WP_000063142.1	BrnA antitoxin family protein	Antitoxin
BCB01_RS19305 (3980820)	3980820..3981107	-	288	WP_001131963.1	BrnT family toxin	Toxin
BCB01_RS19310 (3981517)	3981517..3983358	+	1842	WP_000974637.1	nuclease-related domain-containing DEAD/DEAH box helicase	-
BCB01_RS19315 (3983971)	3983971..3984546	+	576	WP_000593784.1	restriction endonuclease subunit S	-
BCB01_RS19320 (3984551)	3984551..3985804	+	1254	WP_001206755.1	N-6 DNA methylase	-
BCB01_RS23305 (3985836)	3985836..3985970	-	135	WP_001055725.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11294.80 Da
• Isoelectric Point: 9.5894

>T203043 WP_001131963.1 NZ_CP075120:c3981107-3980820 [Salmonella enterica subsp. enterica serovar Enteritidis]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T203043 NZ_CP099868:2453947-2454333 [Escherichia albertii]
ATGATTTGGGTCAGTGCACAGGAGGTTATTGCCTTCCACGATCGAATTTTGCAACGTTTTCCCGGTGTGGCGGGCCTGAC
TGACCCCGGCAGAGCACAGGCGTTGATTTATCGCGTGCAGAATCGTGTTCACTACGAGGGCGTTACTGACCTGTTTGAAC
TGGCAGCAACCTACTGGGTTGCCATCGCTCGCGGTCACATCTTTCATGATGGTAATAAACGTACCGCATTTTTCGTTACT
ATGACCTTTCTTTGGCGCAATGGCATCCGTATCTGTGACGTTGATAACTCGCTGGAGAACCTGACTGTCGAAGCGGCTAC
CGGAGAAAAAACGGTCGGTCAATTAGCGCGATGTTTACGTGAACGAGTGGATAGTTCCGGGAATTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11373.96 Da
• Isoelectric Point: 10.1293

>AT203043 WP_000063142.1 NZ_CP075120:c3980833-3980531 [Salmonella enterica subsp. enterica serovar Enteritidis]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT203043 NZ_CP099868:2453729-2453950 [Escherichia albertii]
ATGCAAACTTACACTTCCACACAAGCCAGAGCCAATATTGCCACTGTACTGGATACCGCGTTGAATGGTGAGCCAGTTGA
AATCACCCGTCGCGACGGCAGCTCTGCCGTGCTCATTAGTAAAGCCGCATTCGAGGCGTATCAAAATGCGAAACTGGACG
CGGAATTTGATGCCATCATGCAACGTCATGAACACACAATTGAAGCGTTGACTGATCGATGA

Structures

Toxin

• PDB: 7VD7

Antitoxin

• AlphaFold DB: A0A4D6PB01