Detailed information of TA system TA203016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 3980855..3981431
• Replicon: chromosome
• Accession: NZ_CP075118
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain CFSAN051890

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: M7RG88
• Locus tag: BCB09_RS19305
• Protein ID: WP_001131963.1
• Coordinates: 3981144..3981431 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: B5R9R5
• Locus tag: BCB09_RS19300
• Protein ID: WP_000063142.1
• Coordinates: 3980855..3981157 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCB09_RS19285 (3977365)	3977365..3979515	+	2151	WP_000379928.1	pyruvate/proton symporter BtsT	-
BCB09_RS19290 (3979610)	3979610..3979813	+	204	WP_000460616.1	YbdD/YjiX family protein	-
BCB09_RS19295 (3979824)	3979824..3980780	+	957	WP_000187843.1	GTPase	-
BCB09_RS19300 (3980855)	3980855..3981157	-	303	WP_000063142.1	BrnA antitoxin family protein	Antitoxin
BCB09_RS19305 (3981144)	3981144..3981431	-	288	WP_001131963.1	BrnT family toxin	Toxin
BCB09_RS19310 (3981855)	3981855..3983696	+	1842	WP_000974637.1	nuclease-related domain-containing DEAD/DEAH box helicase	-
BCB09_RS19315 (3984309)	3984309..3984884	+	576	WP_000593784.1	restriction endonuclease subunit S	-
BCB09_RS19320 (3984889)	3984889..3986142	+	1254	WP_001206755.1	N-6 DNA methylase	-
BCB09_RS23295 (3986174)	3986174..3986308	-	135	WP_001055725.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11294.80 Da
• Isoelectric Point: 9.5894

>T203016 WP_001131963.1 NZ_CP075118:c3981431-3981144 [Salmonella enterica subsp. enterica serovar Enteritidis]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T203016 NZ_CP099834:786725-787135 [Klebsiella quasipneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTCCACGGCGTTGTAGCGGC
GCTGGTGCTGCTGGTTCCCTGGCCGTTAAGCTACACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTTGACTGTG
TGCGCAGCCAGCGACGGATTCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGGCTGCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTCATCAACAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGACGCGG
TCAGCATCTGTGGCTGGCGGCGGACAGCATGGATCCCGGAGAGTGGCGGGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11373.96 Da
• Isoelectric Point: 10.1293

>AT203016 WP_000063142.1 NZ_CP075118:c3981157-3980855 [Salmonella enterica subsp. enterica serovar Enteritidis]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT203016 NZ_CP099834:786478-786744 [Klebsiella quasipneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 7VD7

Antitoxin

• AlphaFold DB: A0A4D6PB01