Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4145046..4145622 Replicon chromosome
Accession NZ_CP075113
Organism Salmonella enterica subsp. enterica serovar Dublin strain CFSAN059898

Toxin (Protein)


Gene name shpA Uniprot ID M7RG88
Locus tag CCO47_RS20295 Protein ID WP_001131963.1
Coordinates 4145335..4145622 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A5U1K3M6
Locus tag CCO47_RS20290 Protein ID WP_000063141.1
Coordinates 4145046..4145348 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCO47_RS20275 (4141556) 4141556..4143706 + 2151 WP_000379928.1 pyruvate/proton symporter BtsT -
CCO47_RS20280 (4143801) 4143801..4144004 + 204 WP_000460616.1 YbdD/YjiX family protein -
CCO47_RS20285 (4144015) 4144015..4144971 + 957 WP_000187843.1 GTPase -
CCO47_RS20290 (4145046) 4145046..4145348 - 303 WP_000063141.1 BrnA antitoxin family protein Antitoxin
CCO47_RS20295 (4145335) 4145335..4145622 - 288 WP_001131963.1 BrnT family toxin Toxin
CCO47_RS20300 (4146046) 4146046..4147887 + 1842 WP_000974635.1 nuclease-related domain-containing DEAD/DEAH box helicase -
CCO47_RS20305 (4148500) 4148500..4149075 + 576 WP_000593782.1 restriction endonuclease subunit S -
CCO47_RS20310 (4149506) 4149506..4150332 + 827 Protein_3998 SAM-dependent methyltransferase -
CCO47_RS24290 (4150364) 4150364..4150498 - 135 WP_001055725.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11294.80 Da        Isoelectric Point: 9.5894

>T202945 WP_001131963.1 NZ_CP075113:c4145622-4145335 [Salmonella enterica subsp. enterica serovar Dublin]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T202945 NZ_CP099754:3047081-3047575 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11431.99 Da        Isoelectric Point: 9.8948

>AT202945 WP_000063141.1 NZ_CP075113:c4145348-4145046 [Salmonella enterica subsp. enterica serovar Dublin]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRDKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Download         Length: 303 bp

>AT202945 NZ_CP099754:3046791-3047084 [Escherichia coli]
ATGGCAGCAGCCAATAACATTCCAGCCAGGCGCGAATCTTTAAACTTGCGCATAAAACCCGCAGAACGCGATCTTATAGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCTCCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VD7

Antitoxin

Source ID Structure
AlphaFold DB A0A5U1K3M6

References